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• W243553406 abstract "Extracts from Phaseolus vulgaris (Fabaceae) were studied for their potential effects on the metabolism of osteoblastic cells. Results indicate that the hydro-ethanolic and acetonic extract stimulates the proliferation of ROS cells. The stimulative effects induced by hydro-ethanolic extract persist even in the presence of an inhibitor of estrogenic receptor such as Tamoxifen. The addition of Tamoxifen in the culture medium inhibits the effects induced by acetonic extract of Phaseolus vulgaris for 10-6 and 10-5 g/ml doses of the extract. For higher doses of extract such as 10-4 and 10-3 g/ml, the stimulative effects of acetonic extract is not affected by Tamoxifen. When the Tamoxifen is added to the media before extracts, effects induced by plant extracts persist only at 10-3 g/ml. The effects disappear at 10-6, 10-5 and 10-4 g/ml. The hydro-ethanolic extract stimulates the differentiation of osteoblastic ROS cells whereas the acetonic extract has no such effect. The effects induced by the hydro-ethanolic extract disappear in the presence of Tamoxifen. Our results indicate that extracts of Phaseolus vulgaris stimulate both the proliferation and the differentiation of osteoblastic cells. These actions could be due, among other reasons, to phyto-estrogens in the extract acting, at least partially, on the estrogens receptors. These molecules may also act through mechanisms where estrogen receptors are not involved. Our results suggest the presence of non-estrogenic substances and/or acting through mechanisms different from those of the estrogen receptors." @default.
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• W243553406 date "2013-01-01" @default.
• W243553406 modified "2023-09-23" @default.
• W243553406 title "Stimulating effects of hydro-ethanolic and acetonic extracts of Phaseolus vulgaris (Fabaceae) on proliferation and differentiation of osteoblastic cells in vitro" @default.
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