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- W2435648310 abstract "MicroRNA (miRNA) analysis has evolved over the past two decades to become a highly specialized field with broad-reaching applications across a multitude of diseases and cellular processes. The choice of an applicable approach for miRNA quantification will depend on a variety of factors such as cost, time constraints, and throughput. Here, we describe the methods of total RNA isolation, AGO2-bound RNA isolation, miRNA polyadenylation, miRNA-cDNA synthesis, and quantitative real-time polymerase chain reaction for the detection of known miRNAs in cultured cells or xenograft tissues of breast cancer." @default.
- W2435648310 created "2016-06-24" @default.
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- W2435648310 date "2016-01-01" @default.
- W2435648310 modified "2023-10-16" @default.
- W2435648310 title "Detection of miRNA in Cultured Cells or Xenograft Tissues of Breast Cancer" @default.
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- W2435648310 doi "https://doi.org/10.1007/978-1-4939-3444-7_7" @default.
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