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- W2435671837 abstract "A five min. incubation of peripheral blood mononuclear cells (PBMN) with either phytohaemagglutinin (PHA) or concanavalin A (ConA) resulted in distinct subcellular redistribution patterns of phosphatidylinositol 4,5-bisphosphate phospholipase C [PtdIns(4,5)P2-PLC] and myo-inositol 1,4,5-trisphosphate monophosphatase [Ins(1,4,5)P3-monophosphatase] activities. When compared to control cells, PHA-treated PBMN cells displayed a significant increase of PtdIns(4,5)P2-PLC and Ins(1,4,5)P3-monophosphatase relative specific activities in the nuclear fraction along with an increment (D) in enzyme amount of 6.5% and 7.3%, respectively. Incubation with B66.6, an anti-CD4 monoclonal antibody (Mab) which specifically activates CD4(+)-T cells in the absence of any other stimuli, also induced changes of these activities in the nuclear fraction, thus mimicking the effect of PHA observed in helper T cell subpopulation. No changes were detected after incubation of PBMN cells with the non mitogenic anti-CD4 MAb 101-69, or with an anti-CD3 MAb which activates T cells only in the presence of a second stimulus. On the other hand, after incubation with ConA, PtdIns(4,5)P2-PLC relative specific activity was enhanced in the microsomal fraction while the Ins(1,4,5)P3-monophosphatase activity increased in both nuclear and microsomal fractions and decreased in cytosol. An increment D of 4.6% and 10.9% for PtdIns(4,5)P2-PLC and Ins(1,4,5)P3-monophosphatase, respectively, was measured in the microsomal fraction. Only after three days of incubation with a mitogenic anti-CD2 MAb Lau-2.1.2, the PtdIns(4,5)P2-PLC activity increased in the particulate fraction of PBMN similar to ConA treatment." @default.
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- W2435671837 date "1993-02-01" @default.
- W2435671837 modified "2023-09-23" @default.
- W2435671837 title "Lectins and anti-T monoclonal antibodies-induced changes of second messengers generating enzymes in human peripheral blood mononuclear cells." @default.
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