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- W2437876786 abstract "ABSTRACT The roles of the head and tail domains of α-internexin, a type IV neuronal intermediate filament protein, in its self-assembly and coassemblies with neurofilament triplet proteins, were examined by transient transfections with deletion mutants in a non-neuronal cell line lacking an endogenous cytoplasmic intermediate filament network. The results from the self-assembly studies showed that the head domain was essential for α-internexin’s ability to self-assemble into a filament network and the tail domain was important for establishing a proper filament network. The data from the coassembly studies demonstrated that α- internexin interacted differentially with the neurofilament triplet protein subunits. Wild-type NF-L or NF-M, but not NF-H, was able to complement and form a normal filament network with the tailless α-internexin mutant, the α- internexin head-deletion mutant, or the α-internexin mutant missing the entire tail and some amino-terminal portion of the head domain. In contrast, neither the tailless NF-L mutant nor the NF-L head-deletion mutant was able to form a normal filament network with any of these α- internexin deletion mutants. However, coassembly of the tailless NF-M mutant with the α-internexin head-deletion mutant and coassembly of the NF-M head-deletion mutant with the tailless α-internexin mutant resulted in the formation of a normal filament network. Thus, the coassembly between α-internexin and NF-M exhibits some unique characteristics previously not observed with other intermediate filament proteins: only one intact tail and one intact head are required for the formation of a normal filament network, and they can be present within the same partner or separately in two partners." @default.
- W2437876786 created "2016-06-24" @default.
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- W2437876786 date "1998-02-01" @default.
- W2437876786 modified "2023-10-02" @default.
- W2437876786 title "Roles of head and tail domains in α-internexin’s self-assembly and coassembly with the neurofilament triplet proteins" @default.
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- W2437876786 doi "https://doi.org/10.1242/jcs.111.3.321" @default.
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