FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2439507130> ?p ?o ?g. }

• W2439507130 endingPage "2602" @default.
• W2439507130 startingPage "2594" @default.
• W2439507130 abstract "In the present study, we have investigated the mechanism of affinity modulation of alpha IIb beta 3 by chymotrypsin. We first confirmed that alpha-chymotrypsin could activate alpha IIb beta 3 (approximately 7,000 molecules per platelet) without major intracellular signaling. However, we unexpectedly found that high concentrations of amiloride dose-dependently inhibited 125I-fibrinogen binding to the chymotrypsin-treated platelets, as well as the platelet aggregation (IC50 [50% inhibitory concentration] for fibrinogen binding, 530 mumol/L). In contrast, amiloride did not inhibit alpha IIb beta 3 activation induced by anti-alpha IIb beta 3 monoclonal antibody PT25–2 or AP5. To identify the pathway involved, the effects of alteration of Na+ gradient in platelets were examined. Lowering Na+ gradient by replacing extracellular Na+ with tetramethylammonium (TMA) increased the number of activated alpha IIb beta 3 by twofold, as assessed by fibrinogen-binding assay. The incubation of platelets with ouabain, a Na+/K(+)- adenosine triphosphatase (ATPase) inhibitor, further augmented alpha IIb beta 3 activation. These data suggested that a likely candidate for the pathway was Na+/Ca2+ exchanger. At 140 mmol/L [Na+]o, 45Ca2+ influx to the chymotrypsin-treated platelets was twofold greater than that to non-treated platelets. Replacement of Na+ with TMA further increased the Ca2+ influx, and the increase was inhibited by amiloride dose-dependently. 3′,4′-Dichlorobenzamil (DCB) and bepridil, relatively specific inhibitors of Na+/Ca2+ exchanger, also inhibited the chymotrypsin-induced alpha IIb beta 3 activation, and the IC50 values of these inhibitors for fibrinogen binding were 25 mumol/L and 52 mumol/L, respectively. Moreover, platelet aggregation induced by various physiologic agonists was inhibited by DCB or bepridil, while platelet agglutination by ristocetin was not. Our data newly suggest that Na+/Ca2+ exchanger operating in reverse mode may be directly involved in inside-out signaling that activates alpha IIb beta 3." @default.
• W2439507130 created "2016-06-24" @default.
• W2439507130 creator A5011679985 @default.
• W2439507130 creator A5030091429 @default.
• W2439507130 creator A5041425436 @default.
• W2439507130 creator A5043150941 @default.
• W2439507130 creator A5049500146 @default.
• W2439507130 creator A5050278092 @default.
• W2439507130 creator A5055453369 @default.
• W2439507130 creator A5058909601 @default.
• W2439507130 creator A5074835045 @default.
• W2439507130 creator A5086129270 @default.
• W2439507130 date "1996-10-01" @default.
• W2439507130 modified "2023-10-16" @default.
• W2439507130 title "Affinity modulation of the platelet integrin alpha IIb beta 3 by alpha- chymotrypsin: a possible role for Na+/Ca2+ exchanger" @default.
• W2439507130 cites W1499223801 @default.
• W2439507130 cites W1531882358 @default.
• W2439507130 cites W1556263106 @default.
• W2439507130 cites W1562376535 @default.
• W2439507130 cites W1567129527 @default.
• W2439507130 cites W1584713825 @default.
• W2439507130 cites W1601529928 @default.
• W2439507130 cites W1656991593 @default.
• W2439507130 cites W166640503 @default.
• W2439507130 cites W1800008863 @default.
• W2439507130 cites W1847283821 @default.
• W2439507130 cites W1901030926 @default.
• W2439507130 cites W1924175076 @default.
• W2439507130 cites W1983768617 @default.
• W2439507130 cites W1984592529 @default.
• W2439507130 cites W1995025282 @default.
• W2439507130 cites W1996100314 @default.
• W2439507130 cites W1999922759 @default.
• W2439507130 cites W2009967151 @default.
• W2439507130 cites W2012691080 @default.
• W2439507130 cites W2021047502 @default.
• W2439507130 cites W2029231027 @default.
• W2439507130 cites W2054543682 @default.
• W2439507130 cites W2055503182 @default.
• W2439507130 cites W2059201211 @default.
• W2439507130 cites W2060639611 @default.
• W2439507130 cites W2071572294 @default.
• W2439507130 cites W2072914802 @default.
• W2439507130 cites W2076299040 @default.
• W2439507130 cites W207951093 @default.
• W2439507130 cites W2081401313 @default.
• W2439507130 cites W2089562291 @default.
• W2439507130 cites W2090904763 @default.
• W2439507130 cites W2113049699 @default.
• W2439507130 cites W2169030528 @default.
• W2439507130 cites W2225782639 @default.
• W2439507130 cites W2227357774 @default.
• W2439507130 cites W2328200817 @default.
• W2439507130 cites W2329922097 @default.
• W2439507130 cites W2335477357 @default.
• W2439507130 cites W2339246231 @default.
• W2439507130 cites W2344606374 @default.
• W2439507130 cites W2395350197 @default.
• W2439507130 cites W2395417405 @default.
• W2439507130 cites W2397619464 @default.
• W2439507130 cites W2410007954 @default.
• W2439507130 cites W2472510931 @default.
• W2439507130 cites W295448432 @default.
• W2439507130 cites W304878669 @default.
• W2439507130 doi "https://doi.org/10.1182/blood.v88.7.2594.bloodjournal8872594" @default.
• W2439507130 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8839852" @default.
• W2439507130 hasPublicationYear "1996" @default.
• W2439507130 type Work @default.
• W2439507130 sameAs 2439507130 @default.
• W2439507130 citedByCount "15" @default.
• W2439507130 countsByYear W24395071302012 @default.
• W2439507130 crossrefType "journal-article" @default.
• W2439507130 hasAuthorship W2439507130A5011679985 @default.
• W2439507130 hasAuthorship W2439507130A5030091429 @default.
• W2439507130 hasAuthorship W2439507130A5041425436 @default.
• W2439507130 hasAuthorship W2439507130A5043150941 @default.
• W2439507130 hasAuthorship W2439507130A5049500146 @default.
• W2439507130 hasAuthorship W2439507130A5050278092 @default.
• W2439507130 hasAuthorship W2439507130A5055453369 @default.
• W2439507130 hasAuthorship W2439507130A5058909601 @default.
• W2439507130 hasAuthorship W2439507130A5074835045 @default.
• W2439507130 hasAuthorship W2439507130A5086129270 @default.
• W2439507130 hasBestOaLocation W24395071301 @default.
• W2439507130 hasConcept C12554922 @default.
• W2439507130 hasConcept C126322002 @default.
• W2439507130 hasConcept C153911025 @default.
• W2439507130 hasConcept C178790620 @default.
• W2439507130 hasConcept C181199279 @default.
• W2439507130 hasConcept C185592680 @default.
• W2439507130 hasConcept C2777022698 @default.
• W2439507130 hasConcept C2778401398 @default.
• W2439507130 hasConcept C2778937662 @default.
• W2439507130 hasConcept C2779608910 @default.
• W2439507130 hasConcept C2780340462 @default.
• W2439507130 hasConcept C519063684 @default.
• W2439507130 hasConcept C537181965 @default.
• W2439507130 hasConcept C55493867 @default.
• W2439507130 hasConcept C71924100 @default.

• next