Matches in SemOpenAlex for { <https://semopenalex.org/work/W2440228238> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W2440228238 endingPage "F1210" @default.
- W2440228238 startingPage "F1198" @default.
- W2440228238 abstract "Arginine vasopressin (AVP) is a nonapeptide that regulates body fluid and blood pressure homeostasis. We have used expression cloning in the Xenopus laevis oocyte system to identify cDNA clones from a rabbit renal medullary expression library encoding an AVP receptor linked to Ca2+ mobilization. cRNA generated from positive clones conferred upon oocytes the capacity to mobilize intracellular Ca2+ in response to AVP. A cDNA clone encoding a protein of 780 amino acids was isolated, sequenced, and subcloned into an SV40-based expression vector. Expression of the cloned protein [designated the vasopressin-activated, calcium-mobilizing (VACM-1) protein] in COS-1 cells, resulted in increased 125I-labeled AVP binding [dissociation constant (Kd) of approximately 2 nM] and increased AVP-induced mobilization of Ca2+. Importantly, 125I-AVP could be immunoprecipitated both from detergent-solubilized membranes from COS-1 cells expressing VACM-1 protein and from an in vitro translation system, in which VACM-1 protein was synthesized, using antibodies prepared against a synthetic peptide derived from the NH2-terminal sequence of VACM-1. Interestingly, immunohistochemical staining of rabbit kidney sections with this antibody showed specific staining of collecting tubule epithelia. The deduced amino acid sequence is not homologous with any nucleic acid or amino acid sequences reported to date, including those of the V1 and V2 AVP receptors. The VACM-1 protein may represent a novel AVP receptor." @default.
- W2440228238 created "2016-06-24" @default.
- W2440228238 creator A5019709058 @default.
- W2440228238 creator A5022580869 @default.
- W2440228238 creator A5025677580 @default.
- W2440228238 creator A5066806044 @default.
- W2440228238 creator A5067458618 @default.
- W2440228238 creator A5068702730 @default.
- W2440228238 date "1995-06-01" @default.
- W2440228238 modified "2023-10-15" @default.
- W2440228238 title "Expression cloning of an AVP-activated, calcium-mobilizing receptor from rabbit kidney medulla" @default.
- W2440228238 doi "https://doi.org/10.1152/ajprenal.1995.268.6.f1198" @default.
- W2440228238 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7611460" @default.
- W2440228238 hasPublicationYear "1995" @default.
- W2440228238 type Work @default.
- W2440228238 sameAs 2440228238 @default.
- W2440228238 citedByCount "20" @default.
- W2440228238 countsByYear W24402282382016 @default.
- W2440228238 countsByYear W24402282382019 @default.
- W2440228238 countsByYear W24402282382020 @default.
- W2440228238 crossrefType "journal-article" @default.
- W2440228238 hasAuthorship W2440228238A5019709058 @default.
- W2440228238 hasAuthorship W2440228238A5022580869 @default.
- W2440228238 hasAuthorship W2440228238A5025677580 @default.
- W2440228238 hasAuthorship W2440228238A5066806044 @default.
- W2440228238 hasAuthorship W2440228238A5067458618 @default.
- W2440228238 hasAuthorship W2440228238A5068702730 @default.
- W2440228238 hasConcept C104317684 @default.
- W2440228238 hasConcept C153911025 @default.
- W2440228238 hasConcept C167625842 @default.
- W2440228238 hasConcept C170493617 @default.
- W2440228238 hasConcept C187882448 @default.
- W2440228238 hasConcept C515207424 @default.
- W2440228238 hasConcept C55493867 @default.
- W2440228238 hasConcept C86803240 @default.
- W2440228238 hasConceptScore W2440228238C104317684 @default.
- W2440228238 hasConceptScore W2440228238C153911025 @default.
- W2440228238 hasConceptScore W2440228238C167625842 @default.
- W2440228238 hasConceptScore W2440228238C170493617 @default.
- W2440228238 hasConceptScore W2440228238C187882448 @default.
- W2440228238 hasConceptScore W2440228238C515207424 @default.
- W2440228238 hasConceptScore W2440228238C55493867 @default.
- W2440228238 hasConceptScore W2440228238C86803240 @default.
- W2440228238 hasIssue "6" @default.
- W2440228238 hasLocation W24402282381 @default.
- W2440228238 hasLocation W24402282382 @default.
- W2440228238 hasOpenAccess W2440228238 @default.
- W2440228238 hasPrimaryLocation W24402282381 @default.
- W2440228238 hasRelatedWork W1481240978 @default.
- W2440228238 hasRelatedWork W1786536704 @default.
- W2440228238 hasRelatedWork W1968548365 @default.
- W2440228238 hasRelatedWork W1998618531 @default.
- W2440228238 hasRelatedWork W1999890227 @default.
- W2440228238 hasRelatedWork W2013783064 @default.
- W2440228238 hasRelatedWork W2015157953 @default.
- W2440228238 hasRelatedWork W2040457845 @default.
- W2440228238 hasRelatedWork W2052370395 @default.
- W2440228238 hasRelatedWork W2063806338 @default.
- W2440228238 hasVolume "268" @default.
- W2440228238 isParatext "false" @default.
- W2440228238 isRetracted "false" @default.
- W2440228238 magId "2440228238" @default.
- W2440228238 workType "article" @default.