Matches in SemOpenAlex for { <https://semopenalex.org/work/W2440756063> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2440756063 abstract "To investigate the apoptosis inducing effects of ponicidin (PON) on leukemic K562 cells and its mechanisms of action.K562 cells in culture medium in vitro were given different concentrations of PON (10-50 micromol x L(-1)) for 24, 48 and 72 h. The inhibitory rate of the cells was measured by MTT assay, cell apoptotic rates were detected by flow cytometry (FCM) using Annexin V staining after K562 cells were treated with different concentrations of PON for 72 hours, and cell morphology was observed by Wright-Giemsa staining. Western blot was used to detect caspase-3 and poly(ADP-ribose) polymerase (PARP) expression, and the protein levels in mitogen-activated protein kinase signaling pathways (MAPKs, p-P38, p-ERK and p-JNK) as well as p-AKT and p-P85 in PI3K/AKT signaling pathways were also detected.PON (over 30 micromol x L(-1)) could inhibit the growth of K562 cells in both time- and dose-dependent manner. FCM analysis revealed that apoptotic cells were gradually increased in a dose-dependent manner after treatment for 72 hours, and that marked morphological changes of cell apoptosis such as condensation of chromatin was clearly observed by Wright-Giemsa staining after treatment by 50 micromol x L(-1) PON. Western blot showed cleavage of the caspase-3 zymogen protein (32 kD), with the appearance of its 17 kD subunit, and a cleaved 89 kD fragment of 116 kD PARP was also found. Furthermore, Western blotting also showed that expression of p-AKT and p-P85 in PI3K/AKT signaling pathways was downregulated dramatically whereas the expression of p-P38 as well as p-ERK and p-JNK remained unchanged after the cells were treated by PON for 48 h.The results demonstrate that PON exhibits in vitro anti-leukemia effect by induction of apoptosis in K562 cells, and that PON induced apoptosis in K562 cells mainly related to activation of caspase-3 as well as inactivation of PI3K/AKT signaling pathway via down regulation of the expression of p-AKT and p-P85 protein levels. These results provide strong laboratory evidence for further anti-leukemia trials of PON." @default.
- W2440756063 created "2016-06-24" @default.
- W2440756063 creator A5009067006 @default.
- W2440756063 creator A5014708973 @default.
- W2440756063 creator A5019627817 @default.
- W2440756063 creator A5028313744 @default.
- W2440756063 creator A5031468074 @default.
- W2440756063 creator A5032219633 @default.
- W2440756063 creator A5047424772 @default.
- W2440756063 creator A5059900841 @default.
- W2440756063 creator A5060808786 @default.
- W2440756063 creator A5072362529 @default.
- W2440756063 creator A5089621082 @default.
- W2440756063 date "2010-08-15" @default.
- W2440756063 modified "2023-10-16" @default.
- W2440756063 title "Apoptosis inducing effect of ponicidin in leukemia K562 cells and its mechanisms of action" @default.
- W2440756063 doi "https://doi.org/10.4268/cjcmm20101628" @default.
- W2440756063 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21046753" @default.
- W2440756063 hasPublicationYear "2010" @default.
- W2440756063 type Work @default.
- W2440756063 sameAs 2440756063 @default.
- W2440756063 citedByCount "1" @default.
- W2440756063 crossrefType "journal-article" @default.
- W2440756063 hasAuthorship W2440756063A5009067006 @default.
- W2440756063 hasAuthorship W2440756063A5014708973 @default.
- W2440756063 hasAuthorship W2440756063A5019627817 @default.
- W2440756063 hasAuthorship W2440756063A5028313744 @default.
- W2440756063 hasAuthorship W2440756063A5031468074 @default.
- W2440756063 hasAuthorship W2440756063A5032219633 @default.
- W2440756063 hasAuthorship W2440756063A5047424772 @default.
- W2440756063 hasAuthorship W2440756063A5059900841 @default.
- W2440756063 hasAuthorship W2440756063A5060808786 @default.
- W2440756063 hasAuthorship W2440756063A5072362529 @default.
- W2440756063 hasAuthorship W2440756063A5089621082 @default.
- W2440756063 hasConcept C104317684 @default.
- W2440756063 hasConcept C153911025 @default.
- W2440756063 hasConcept C182979987 @default.
- W2440756063 hasConcept C184235292 @default.
- W2440756063 hasConcept C185592680 @default.
- W2440756063 hasConcept C190283241 @default.
- W2440756063 hasConcept C2776415932 @default.
- W2440756063 hasConcept C552990157 @default.
- W2440756063 hasConcept C553184892 @default.
- W2440756063 hasConcept C55493867 @default.
- W2440756063 hasConcept C57074206 @default.
- W2440756063 hasConcept C75217442 @default.
- W2440756063 hasConcept C82381507 @default.
- W2440756063 hasConcept C86554907 @default.
- W2440756063 hasConcept C86803240 @default.
- W2440756063 hasConcept C88634738 @default.
- W2440756063 hasConcept C95444343 @default.
- W2440756063 hasConceptScore W2440756063C104317684 @default.
- W2440756063 hasConceptScore W2440756063C153911025 @default.
- W2440756063 hasConceptScore W2440756063C182979987 @default.
- W2440756063 hasConceptScore W2440756063C184235292 @default.
- W2440756063 hasConceptScore W2440756063C185592680 @default.
- W2440756063 hasConceptScore W2440756063C190283241 @default.
- W2440756063 hasConceptScore W2440756063C2776415932 @default.
- W2440756063 hasConceptScore W2440756063C552990157 @default.
- W2440756063 hasConceptScore W2440756063C553184892 @default.
- W2440756063 hasConceptScore W2440756063C55493867 @default.
- W2440756063 hasConceptScore W2440756063C57074206 @default.
- W2440756063 hasConceptScore W2440756063C75217442 @default.
- W2440756063 hasConceptScore W2440756063C82381507 @default.
- W2440756063 hasConceptScore W2440756063C86554907 @default.
- W2440756063 hasConceptScore W2440756063C86803240 @default.
- W2440756063 hasConceptScore W2440756063C88634738 @default.
- W2440756063 hasConceptScore W2440756063C95444343 @default.
- W2440756063 hasLocation W24407560631 @default.
- W2440756063 hasLocation W24407560632 @default.
- W2440756063 hasOpenAccess W2440756063 @default.
- W2440756063 hasPrimaryLocation W24407560631 @default.
- W2440756063 hasRelatedWork W2048391776 @default.
- W2440756063 hasRelatedWork W2359728754 @default.
- W2440756063 hasRelatedWork W2383576447 @default.
- W2440756063 hasRelatedWork W2383837582 @default.
- W2440756063 hasRelatedWork W2384075995 @default.
- W2440756063 hasRelatedWork W2387677605 @default.
- W2440756063 hasRelatedWork W2417585768 @default.
- W2440756063 hasRelatedWork W2463535394 @default.
- W2440756063 hasRelatedWork W264423964 @default.
- W2440756063 hasRelatedWork W3147561810 @default.
- W2440756063 isParatext "false" @default.
- W2440756063 isRetracted "false" @default.
- W2440756063 magId "2440756063" @default.
- W2440756063 workType "article" @default.