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- W244083005 abstract "Polyphenols are a group of compounds that are widely spread in the plant kingdom. These compounds are of great interest for researchers because of their antioxidant properties that are important for both plant life and human health. Sample preparation is a crucial step for the determination of phenolic compounds and their metabolites in biological samples. Methods for isolation and preconcentration of these compounds from human urine matrices by using C-18 HyperSepTM tips associated with micro-solid phase extraction (micro-SPE) technique have been tested in this study. A broad range of polyphenols, namely; phenolic acid, flavonol and anthocyanin standards were separated and identified by Reversed Phase High Temperature Liquid Chromatography (RP-HTLC) coupled to Ultra Violet/ Visible Light (UV/VIS) and PhotoDiode Array detector (PDA). The RP-HTLC-method has been validated; the limit of detection for the phenolic acids ranged between 0.15 and 0.76 μgmL-1 and the calibration curves showed excellent linearity all with R2 > 0.99. The precision of the overall analytical procedure was estimated as the relative standard deviations (RSD %) through repeating the analysis seven times in one day. The RSDs for all retention times were < 1 % and for all peak areas, < 10 %. The Micro-SPE methods studied showed poor recovery which was at best 25 % recovery of vanillic acid and 27 % recovery of 3, 4-dihydroxyphenylacetic acid. (Less)" @default.
- W244083005 created "2016-06-24" @default.
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- W244083005 date "2013-01-01" @default.
- W244083005 modified "2023-09-26" @default.
- W244083005 title "Development of an analytical method for isolation and identification of phenolic compounds in human urine samples" @default.
- W244083005 hasPublicationYear "2013" @default.
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