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- W2441946136 abstract "In situ hybridization (ISH) is employed principally to detect mRNA within cells and tissues. ISH begins with the synthesis of a nucleic acid probe complementary in sequence to a cellular target. Through incorporation of a radioactive nucleotide into the probe, a cellular target may be visualized using autoradiography. A nonradioactive variation of this technique utilizing digoxigenin has been championed by some researchers, but our experience has been that radioactive probes produce more reliable and accurate results. Although not a difficult technique, ISH incorporates many steps, which must be performed with care and precision to obtain useful results." @default.
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- W2441946136 date "2003-11-14" @default.
- W2441946136 modified "2023-09-23" @default.
- W2441946136 title "In Situ Hybridization" @default.
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- W2441946136 doi "https://doi.org/10.1385/1-59259-087-x:223" @default.
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