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- W244823478 abstract "The plasmid carrying chicken interleukin-2(IL-2) gene was constructed and used to prepare DNA-chitosan nanoparticles by complex coacervation process.The important parameters of the nanoparticles were investigated.The nanoparticles synthesized were globule and the diameter ranged from 50 to 500 nm.The nanoparticles were positively charged with a zeta potential of +17.8 mV.DNA counted for 40.2% of the weight of the nanoparticles.Enzyme protection test,stability test and in vitro release test showed that the nanoparticles prepared were stable at pH 6.0 and pH 7.4,and could protect the encapsulated plasmid DNA from nuclease degradation at the nuclease concentration of 0.6～0.8 U/mL.Df-1 cells were transfected by nanoparticles,the result of indirect immuno-fluorescence test demonstrated that the nanoparticles could take the plasmid DNA into cells and the exogenous gene could be expressed by the cells.The transfection efficiency was 0.2% studied by flow cytometry,higher than that of naked DNA transfection control.It demonstrated that encapsulating into nanoparticles can improve the transfection efficiency of DNA." @default.
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- W244823478 date "2009-01-01" @default.
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- W244823478 title "Preparation of chicken interleukin-2 (IL-2) DNA-chitosan nanoparticles and its transfection in vitro." @default.
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