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- W245391626 abstract "Publisher Summary This chapter explores the organization of immunoglobulin kappa light chain genes in germ line and somatic cells. In an experiment described in the chapter, the organization of Ig genes in several tissues and most importantly in the germ line was studied. Mouse sperm and embryo DNA were digested with several restriction enzymes and the resulting fragments were analyzed for their content of kappa light chain sequences by hybridization techniques. A kappa light chain probe specific for the constant region yielded the same restriction map for sperm and embryo DNA and a probe specific for a kappa variable region sequence (MOPC 167) revealed several Vk genes located on the same restriction fragments in sperm and embryo DNA. Hybridization with v and c region probes also revealed differences in the intensity of the corresponding bands on Southern gels. These results are also discussed in the light of different hypotheses that have been put forward to explain the origin of antibody diversity." @default.
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- W245391626 date "1979-01-01" @default.
- W245391626 modified "2023-09-27" @default.
- W245391626 title "ORGANIZATION OF IMMUNOGLOBULIN KAPPA LIGHT CHAIN GENES IN GERM LINE AND SOMATIC CELLS" @default.
- W245391626 doi "https://doi.org/10.1016/b978-0-12-604450-8.50066-3" @default.
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