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- W2460094535 abstract "Sarcomere length (SL) variation in skeletal muscle is known to have significant functional consequences. While numerous studies have quantified variation at the single fiber and myofibrillar levels, surprisingly, it has not been systematically quantified throughout a whole muscle. To address this, we developed an automated device to systematically sample sarcomere length across and along thick longitudinal muscle sections using laser diffraction. PURPOSE: To determine the sarcomere length variation along and across whole rat tibialis anterior (TA) muscles. METHODS: We incorporated a motorized stage into a standard laser diffraction system such that a slide-mounted muscle section could be translated in the horizontal plane relative to a vertical laser path. Custom Matlab software controlled the stage, recorded the diffraction pattern, and automatically determined SL. TA muscle samples from Sprague-Dawley rats (n=4) were 1) fixed on bone in 10% phosphate-buffered formalin at either neutral joint angles (resting length) or with the ankles fully plantar flexed (stretched), 2) dissected and cryo-sectioned at 100 μm thickness, and 3) slide-mounted to produce sagittal plane, longitudinal sections. We scanned sections in 400 μm increments and analyzed two and three-dimensional patterns of SL variation in muscle. RESULTS: We measured an average of 27,618 individual SL values per muscle, equivalent to sampling 72% of the entire muscle volumes. Average SL for the TA muscles at resting length was 2.38±0.07 μm, (mean ± sd) and coefficient of variation was 6.70±0.93 %. Average SL for the stretched TA muscles was 2.89±0.10 μm, and coefficient of variation was 7.00±0.63 %, indicating that stretch did not decrease SL variation as is observed in single fibers. SL varied most significantly between superficial and deep halves of the muscle, with an average difference of 0.21±0.03 μm. CONCLUSION: We successfully measured SL across an entire muscle. Although the degree of SL variability in rat TA muscles is low, the variation is not random but systematically distributed in muscle, suggesting that estimates of average muscle SL may be biased if samples are not uniformly distributed. Supported by NIH Grant P30 AR-058878-02, R24HD050837 and the Department of Veterans Affairs." @default.
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- W2460094535 date "2015-05-01" @default.
- W2460094535 modified "2023-09-27" @default.
- W2460094535 title "Systematic Sarcomere Length Variation Across an Entire Skeletal Muscle" @default.
- W2460094535 doi "https://doi.org/10.1249/01.mss.0000476673.60159.14" @default.
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