Matches in SemOpenAlex for { <https://semopenalex.org/work/W2460408478> ?p ?o ?g. }
- W2460408478 endingPage "52117" @default.
- W2460408478 startingPage "52104" @default.
- W2460408478 abstract "High-risk human papillomaviruses (hr HPVs) may cause various human cancers and associated premalignant lesions. Transformation of the host cells is triggered by overexpression of the viral oncogenes E6 and E7 that deregulate the cell cycle and induce chromosomal instability. This process is accompanied by hypermethylation of distinct CpG sites resulting in silencing of tumor suppressor genes, inhibition of the viral E2 mediated control of E6 and E7 transcription as well as deregulated expression of host cell microRNAs. Therefore, we hypothesized that treatment with demethylating agents might restore those regulatory mechanisms. Here we show that treatment with 5-aza-2'-deoxycytidine (DAC) strongly decreases the expression of E6 and E7 in a panel of HPV-transformed cervical cancer and head and neck squamous cell carcinoma cell lines. Reduction of E6 and E7 further resulted in increased target protein levels including p53 and p21 reducing the proliferation rates and colony formation abilities of the treated cell lines. Moreover, DAC treatment led to enhanced expression of tumor the suppressive miRNA-375 that targets and degrades E6 and E7 transcripts. Therefore, we suggest that DAC treatment of HPV-associated cancers and respective precursor lesions may constitute a targeted approach to subvert HPV oncogene functions that deserves testing in clinical trials." @default.
- W2460408478 created "2016-07-22" @default.
- W2460408478 creator A5005941126 @default.
- W2460408478 creator A5007438121 @default.
- W2460408478 creator A5024936471 @default.
- W2460408478 creator A5033842027 @default.
- W2460408478 creator A5039048168 @default.
- W2460408478 creator A5050401175 @default.
- W2460408478 creator A5090474147 @default.
- W2460408478 creator A5044225530 @default.
- W2460408478 date "2016-07-16" @default.
- W2460408478 modified "2023-09-26" @default.
- W2460408478 title "5-aza-2′-deoxycytidine (DAC) treatment downregulates the HPV E6 and E7 oncogene expression and blocks neoplastic growth of HPV-associated cancer cells" @default.
- W2460408478 cites W1485564559 @default.
- W2460408478 cites W1510715774 @default.
- W2460408478 cites W1520483630 @default.
- W2460408478 cites W1526266797 @default.
- W2460408478 cites W1528346173 @default.
- W2460408478 cites W1529128810 @default.
- W2460408478 cites W1633239216 @default.
- W2460408478 cites W1672426070 @default.
- W2460408478 cites W1773758992 @default.
- W2460408478 cites W1799584846 @default.
- W2460408478 cites W1836001664 @default.
- W2460408478 cites W1933052494 @default.
- W2460408478 cites W1945270539 @default.
- W2460408478 cites W1964025444 @default.
- W2460408478 cites W1964783528 @default.
- W2460408478 cites W1971054810 @default.
- W2460408478 cites W1981991171 @default.
- W2460408478 cites W1983023920 @default.
- W2460408478 cites W1984807040 @default.
- W2460408478 cites W2002936023 @default.
- W2460408478 cites W2009504586 @default.
- W2460408478 cites W2023942950 @default.
- W2460408478 cites W2024088186 @default.
- W2460408478 cites W2024202567 @default.
- W2460408478 cites W2037710811 @default.
- W2460408478 cites W2038500217 @default.
- W2460408478 cites W2039382131 @default.
- W2460408478 cites W2041959453 @default.
- W2460408478 cites W2047816695 @default.
- W2460408478 cites W2050847569 @default.
- W2460408478 cites W2054206673 @default.
- W2460408478 cites W2055596206 @default.
- W2460408478 cites W2057241466 @default.
- W2460408478 cites W2065528684 @default.
- W2460408478 cites W2066332001 @default.
- W2460408478 cites W2072667482 @default.
- W2460408478 cites W2077155376 @default.
- W2460408478 cites W2081116295 @default.
- W2460408478 cites W2081134321 @default.
- W2460408478 cites W2081784458 @default.
- W2460408478 cites W2083459920 @default.
- W2460408478 cites W2089801128 @default.
- W2460408478 cites W2093815632 @default.
- W2460408478 cites W2095317270 @default.
- W2460408478 cites W2097322288 @default.
- W2460408478 cites W2098765233 @default.
- W2460408478 cites W2103351598 @default.
- W2460408478 cites W2106401558 @default.
- W2460408478 cites W2109118354 @default.
- W2460408478 cites W2109710744 @default.
- W2460408478 cites W2114676099 @default.
- W2460408478 cites W2116821087 @default.
- W2460408478 cites W2123179912 @default.
- W2460408478 cites W2123851279 @default.
- W2460408478 cites W2131459766 @default.
- W2460408478 cites W2132384241 @default.
- W2460408478 cites W2139975735 @default.
- W2460408478 cites W2140814572 @default.
- W2460408478 cites W2145750252 @default.
- W2460408478 cites W2148731534 @default.
- W2460408478 cites W2149704987 @default.
- W2460408478 cites W2149838021 @default.
- W2460408478 cites W2151501050 @default.
- W2460408478 cites W2151895234 @default.
- W2460408478 cites W2160566717 @default.
- W2460408478 cites W2160695129 @default.
- W2460408478 cites W2166997624 @default.
- W2460408478 cites W2233579036 @default.
- W2460408478 cites W4232645703 @default.
- W2460408478 doi "https://doi.org/10.18632/oncotarget.10631" @default.
- W2460408478 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5581016" @default.
- W2460408478 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28881717" @default.
- W2460408478 hasPublicationYear "2016" @default.
- W2460408478 type Work @default.
- W2460408478 sameAs 2460408478 @default.
- W2460408478 citedByCount "22" @default.
- W2460408478 countsByYear W24604084782017 @default.
- W2460408478 countsByYear W24604084782018 @default.
- W2460408478 countsByYear W24604084782019 @default.
- W2460408478 countsByYear W24604084782020 @default.
- W2460408478 countsByYear W24604084782021 @default.
- W2460408478 countsByYear W24604084782022 @default.
- W2460408478 countsByYear W24604084782023 @default.
- W2460408478 crossrefType "journal-article" @default.
- W2460408478 hasAuthorship W2460408478A5005941126 @default.