FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2460573777> ?p ?o ?g. }

• W2460573777 abstract "Ketosteroid isomerase (3-oxosteroid Δ(5)-Δ(4)-isomerase, KSI) from Pseudomonas putida catalyzes allylic rearrangement of the 5,6-double bond of Δ(5)-3-ketosteroid to 4,5-position by stereospecific intramolecular transfer of a proton. The active site of KSI is formed by several hydrophobic residues and three catalytic residues (Tyr14, Asp38, and Asp99). In this study, we investigated the role of a hydrophobic Met112 residue near the active site in the catalysis, steroid binding, and stability of KSI. Replacing Met112 with alanine (yields M112A) or leucine (M112L) decreased the kcat by 20- and 4-fold, respectively. Compared with the wild type (WT), M112A and M112L KSIs showed increased KD values for equilenin, an intermediate analogue; these changes suggest that loss of packing at position 112 might lead to unfavorable steroid binding, thereby resulting in decreased catalytic activity. Furthermore, M112A and M112L mutations reduced melting temperature (Tm) by 6.4°C and 2.5°C, respectively. These changes suggest that favorable packing in the core is important for the maintenance of stability in KSI. The M112K mutation decreased kcat by 2000-fold, compared with the WT. In M112K KSI structure, a new salt bridge was formed between Asp38 and Lys112. This bridge could change the electrostatic potential of Asp38, and thereby contribute to the decreased catalytic activity. The M112K mutation also decreased the stability by reducing Tm by 4.1°C. Our data suggest that the Met112 residue may contribute to the catalytic activity and stability of KSI by providing favorable hydrophobic environments and compact packing in the catalytic core." @default.
• W2460573777 created "2016-07-22" @default.
• W2460573777 creator A5003403424 @default.
• W2460573777 creator A5007262375 @default.
• W2460573777 creator A5009094679 @default.
• W2460573777 creator A5019330002 @default.
• W2460573777 creator A5042293749 @default.
• W2460573777 creator A5044698881 @default.
• W2460573777 creator A5074805682 @default.
• W2460573777 date "2016-10-01" @default.
• W2460573777 modified "2023-10-18" @default.
• W2460573777 title "Role of conserved Met112 residue in the catalytic activity and stability of ketosteroid isomerase" @default.
• W2460573777 cites W1539796472 @default.
• W2460573777 cites W1895579007 @default.
• W2460573777 cites W1963979993 @default.
• W2460573777 cites W1965876630 @default.
• W2460573777 cites W1987953658 @default.
• W2460573777 cites W1988480048 @default.
• W2460573777 cites W1989417429 @default.
• W2460573777 cites W2001838626 @default.
• W2460573777 cites W2010738939 @default.
• W2460573777 cites W2017747125 @default.
• W2460573777 cites W2028317271 @default.
• W2460573777 cites W2031219961 @default.
• W2460573777 cites W2032884007 @default.
• W2460573777 cites W2034353029 @default.
• W2460573777 cites W2034704893 @default.
• W2460573777 cites W2034767282 @default.
• W2460573777 cites W2038735502 @default.
• W2460573777 cites W2044473641 @default.
• W2460573777 cites W2057443486 @default.
• W2460573777 cites W2066315695 @default.
• W2460573777 cites W2074786454 @default.
• W2460573777 cites W2081659950 @default.
• W2460573777 cites W2081670113 @default.
• W2460573777 cites W2087199620 @default.
• W2460573777 cites W2091313405 @default.
• W2460573777 cites W2099057076 @default.
• W2460573777 cites W2108921801 @default.
• W2460573777 cites W2109582258 @default.
• W2460573777 cites W2124026197 @default.
• W2460573777 cites W2129247440 @default.
• W2460573777 cites W2133852950 @default.
• W2460573777 cites W2134201060 @default.
• W2460573777 cites W2137945247 @default.
• W2460573777 cites W2140794227 @default.
• W2460573777 cites W2142320009 @default.
• W2460573777 cites W2142579834 @default.
• W2460573777 cites W2154197653 @default.
• W2460573777 cites W2163527448 @default.
• W2460573777 cites W2167857557 @default.
• W2460573777 cites W2168974130 @default.
• W2460573777 cites W2311573227 @default.
• W2460573777 cites W2321456458 @default.
• W2460573777 doi "https://doi.org/10.1016/j.bbapap.2016.06.016" @default.
• W2460573777 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27375051" @default.
• W2460573777 hasPublicationYear "2016" @default.
• W2460573777 type Work @default.
• W2460573777 sameAs 2460573777 @default.
• W2460573777 citedByCount "4" @default.
• W2460573777 countsByYear W24605737772019 @default.
• W2460573777 countsByYear W24605737772021 @default.
• W2460573777 countsByYear W24605737772022 @default.
• W2460573777 crossrefType "journal-article" @default.
• W2460573777 hasAuthorship W2460573777A5003403424 @default.
• W2460573777 hasAuthorship W2460573777A5007262375 @default.
• W2460573777 hasAuthorship W2460573777A5009094679 @default.
• W2460573777 hasAuthorship W2460573777A5019330002 @default.
• W2460573777 hasAuthorship W2460573777A5042293749 @default.
• W2460573777 hasAuthorship W2460573777A5044698881 @default.
• W2460573777 hasAuthorship W2460573777A5074805682 @default.
• W2460573777 hasConcept C104317684 @default.
• W2460573777 hasConcept C143065580 @default.
• W2460573777 hasConcept C161790260 @default.
• W2460573777 hasConcept C181199279 @default.
• W2460573777 hasConcept C185592680 @default.
• W2460573777 hasConcept C187921627 @default.
• W2460573777 hasConcept C2778257825 @default.
• W2460573777 hasConcept C2779856020 @default.
• W2460573777 hasConcept C2781264208 @default.
• W2460573777 hasConcept C2781338088 @default.
• W2460573777 hasConcept C30765947 @default.
• W2460573777 hasConcept C41183919 @default.
• W2460573777 hasConcept C515207424 @default.
• W2460573777 hasConcept C55493867 @default.
• W2460573777 hasConcept C56856141 @default.
• W2460573777 hasConcept C71240020 @default.
• W2460573777 hasConceptScore W2460573777C104317684 @default.
• W2460573777 hasConceptScore W2460573777C143065580 @default.
• W2460573777 hasConceptScore W2460573777C161790260 @default.
• W2460573777 hasConceptScore W2460573777C181199279 @default.
• W2460573777 hasConceptScore W2460573777C185592680 @default.
• W2460573777 hasConceptScore W2460573777C187921627 @default.
• W2460573777 hasConceptScore W2460573777C2778257825 @default.
• W2460573777 hasConceptScore W2460573777C2779856020 @default.
• W2460573777 hasConceptScore W2460573777C2781264208 @default.
• W2460573777 hasConceptScore W2460573777C2781338088 @default.
• W2460573777 hasConceptScore W2460573777C30765947 @default.
• W2460573777 hasConceptScore W2460573777C41183919 @default.
• W2460573777 hasConceptScore W2460573777C515207424 @default.

• next