FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2460912104> ?p ?o ?g. }

• W2460912104 endingPage "41" @default.
• W2460912104 startingPage "32" @default.
• W2460912104 abstract "Ceramide-1-phosphate (C1P) is an important signaling sphingolipid and a metabolite of ceramide. C1P contains an anionic phosphomonoester head group and has been shown to regulate physiological and pathophysiological processes such as cell proliferation, inflammation, apoptosis, phagocytosis, and macrophage chemotaxis. Despite this mechanistic information on its role in intra- and intercellular communication, little information is available on the biophysical properties of C1P in biological membranes and how it interacts with effector proteins. Fluorescently labeled lipids have been a useful tool to understand the membrane behavior properties of lipids such as phosphatidylserine, cholesterol, and some phosphoinositides. However, to the best of our knowledge, fluorescently labeled C1P hasn’t been implemented to investigate its ability to serve as a mimetic of endogenous C1P in cells or untagged C1P in in vitro experiments. Cellular and in vitro assays demonstrate TopFluor-C1P harbors a fluorescent group that is fully buried in the hydrocarbon core and fluoresces across the spectrum of physiological pH values. Moreover, TopFluor-C1P didn’t affect cellular toxicity at concentrations employed, was as effective as unlabeled C1P in recruiting an established protein effector to intracellular membranes, and its subcellular localization recapitulated what is known for endogenous C1P. Notably, the diffusion coefficient of TopFluor-C1P was slower than that of TopFluor-phosphatidylserine or TopFluor-cholesterol in the plasma membrane and similar to that of other fluorescently labeled sphingolipids including ceramide and sphingomyelin. These studies demonstrate that TopFluor-C1P should be a reliable mimetic of C1P to study C1P membrane biophysical properties and C1P interactions with proteins." @default.
• W2460912104 created "2016-07-22" @default.
• W2460912104 creator A5006866639 @default.
• W2460912104 creator A5078195240 @default.
• W2460912104 creator A5080000506 @default.
• W2460912104 date "2016-10-01" @default.
• W2460912104 modified "2023-09-30" @default.
• W2460912104 title "Investigation of the biophysical properties of a fluorescently modified ceramide-1-phosphate" @default.
• W2460912104 cites W1256777839 @default.
• W2460912104 cites W1545297508 @default.
• W2460912104 cites W1554191570 @default.
• W2460912104 cites W1970138343 @default.
• W2460912104 cites W1974606305 @default.
• W2460912104 cites W1978119458 @default.
• W2460912104 cites W1980885060 @default.
• W2460912104 cites W1982277787 @default.
• W2460912104 cites W1983351621 @default.
• W2460912104 cites W1993740563 @default.
• W2460912104 cites W1996870265 @default.
• W2460912104 cites W1998195211 @default.
• W2460912104 cites W2002012958 @default.
• W2460912104 cites W2008931806 @default.
• W2460912104 cites W2009547046 @default.
• W2460912104 cites W2009814603 @default.
• W2460912104 cites W2010093307 @default.
• W2460912104 cites W2019516160 @default.
• W2460912104 cites W2031143643 @default.
• W2460912104 cites W2035353955 @default.
• W2460912104 cites W2040566502 @default.
• W2460912104 cites W2043622799 @default.
• W2460912104 cites W2044097637 @default.
• W2460912104 cites W2045068620 @default.
• W2460912104 cites W2052602536 @default.
• W2460912104 cites W2058188594 @default.
• W2460912104 cites W2066594184 @default.
• W2460912104 cites W2070325049 @default.
• W2460912104 cites W2075419670 @default.
• W2460912104 cites W2092170895 @default.
• W2460912104 cites W2094096164 @default.
• W2460912104 cites W2094272082 @default.
• W2460912104 cites W2095166308 @default.
• W2460912104 cites W2099559490 @default.
• W2460912104 cites W2101040824 @default.
• W2460912104 cites W2101662431 @default.
• W2460912104 cites W2103305118 @default.
• W2460912104 cites W2105646576 @default.
• W2460912104 cites W2116113293 @default.
• W2460912104 cites W2120233956 @default.
• W2460912104 cites W2120478897 @default.
• W2460912104 cites W2122437389 @default.
• W2460912104 cites W2133011371 @default.
• W2460912104 cites W2138459238 @default.
• W2460912104 cites W2145741262 @default.
• W2460912104 cites W2155000549 @default.
• W2460912104 cites W2159516959 @default.
• W2460912104 cites W2221295570 @default.
• W2460912104 cites W2228647364 @default.
• W2460912104 cites W4244540082 @default.
• W2460912104 cites W4255794096 @default.
• W2460912104 doi "https://doi.org/10.1016/j.chemphyslip.2016.06.004" @default.
• W2460912104 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5159338" @default.
• W2460912104 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27318040" @default.
• W2460912104 hasPublicationYear "2016" @default.
• W2460912104 type Work @default.
• W2460912104 sameAs 2460912104 @default.
• W2460912104 citedByCount "4" @default.
• W2460912104 countsByYear W24609121042018 @default.
• W2460912104 countsByYear W24609121042019 @default.
• W2460912104 countsByYear W24609121042021 @default.
• W2460912104 crossrefType "journal-article" @default.
• W2460912104 hasAuthorship W2460912104A5006866639 @default.
• W2460912104 hasAuthorship W2460912104A5078195240 @default.
• W2460912104 hasAuthorship W2460912104A5080000506 @default.
• W2460912104 hasBestOaLocation W24609121041 @default.
• W2460912104 hasConcept C170493617 @default.
• W2460912104 hasConcept C180899940 @default.
• W2460912104 hasConcept C185592680 @default.
• W2460912104 hasConcept C190283241 @default.
• W2460912104 hasConcept C2777851122 @default.
• W2460912104 hasConcept C2778918659 @default.
• W2460912104 hasConcept C2779637612 @default.
• W2460912104 hasConcept C41625074 @default.
• W2460912104 hasConcept C51785407 @default.
• W2460912104 hasConcept C55493867 @default.
• W2460912104 hasConcept C61322309 @default.
• W2460912104 hasConcept C61705674 @default.
• W2460912104 hasConcept C79879829 @default.
• W2460912104 hasConcept C86803240 @default.
• W2460912104 hasConcept C95444343 @default.
• W2460912104 hasConceptScore W2460912104C170493617 @default.
• W2460912104 hasConceptScore W2460912104C180899940 @default.
• W2460912104 hasConceptScore W2460912104C185592680 @default.
• W2460912104 hasConceptScore W2460912104C190283241 @default.
• W2460912104 hasConceptScore W2460912104C2777851122 @default.
• W2460912104 hasConceptScore W2460912104C2778918659 @default.
• W2460912104 hasConceptScore W2460912104C2779637612 @default.
• W2460912104 hasConceptScore W2460912104C41625074 @default.
• W2460912104 hasConceptScore W2460912104C51785407 @default.

• next