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• W2460946396 abstract "A recyclable strategy for the production of high-purity (>95%) galacto-oligosaccharides (GOS) was developed using Kluyveromyces lactis in both the synthesis and purification steps. For the synthesis of GOS, ethanol-permeabilized cells (p-cells) of K. lactis were used because the enhanced permeability facilitated the mass transfer of the substrate and the release of oligosaccharide products. For the purification of GOS, non-permeabilized K. lactis cells (np-cells) were preferred as a result of their intrinsic cell membrane barrier toward GOS, which led to the selective consumption of carbohydrate. In this way, undesired glucose, galactose, and lactose in the raw GOS solution can be completely removed. This strategy is recyclable not only because of the high stability and reusability of p-cells and np-cells but also because the ethanol, which is simultaneously generated during the purification, can be reused for the preparation of p-cells. The strategy proposed in this study is a promising candidate for the efficient production of high-purity GOS." @default.
• W2460946396 created "2016-07-22" @default.
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• W2460946396 date "2016-07-08" @default.
• W2460946396 modified "2023-10-18" @default.
• W2460946396 title "Recyclable Strategy for the Production of High-Purity Galacto-oligosaccharides by <i>Kluyveromyces lactis</i>" @default.
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• W2460946396 doi "https://doi.org/10.1021/acs.jafc.6b01531" @default.
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