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• W2461303667 abstract "The development of a technique to stably integrate exogenous DNA into the germline of Drosophila melanogaster marked a milestone in the ability to study gene function in the fly.On the molecular level germline transformation mainly relies on a particular transposable element, the D. melanogaster P-element. Based on certain features of the P-element, vectors have been designed for diverse applications like gene disruption, chromosome engineering, gene tagging, and inducible gene expression/repression. Despite the fact that an increasing number of other transposons have been utilized for germline transformation of Drosophila most transformation vectors are still P-element based.Technically, microinjection serves as the method of choice to physically introduce transgenes into preblastoderm Drosophila embryos. Besides an appropriate technical equipment including suitable microcapillaries in conjunction with a micromanipulator, a microinjector, and a microscope, proper handling of the Drosophila embryos before and after microinjection is the key step to the generation of transgenic flies. Pioneer work in Drosophila also served as a general guideline for the transformation of other insect species including those with medical and agricultural importance." @default.
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• W2461303667 date "2008-01-01" @default.
• W2461303667 modified "2023-09-26" @default.
• W2461303667 title "The Use of P-Element Transposons to Generate Transgenic Flies" @default.
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• W2461303667 doi "https://doi.org/10.1007/978-1-59745-583-1_4" @default.
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