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- W2461487989 abstract "To investigate the effect of vascular endothelial growth factor(VEGF) gene transfection on tissue vascularization in transplanted site and the proliferation of transplanted hepatocytes.Samples were gained at ten days after the pcDNA3 VEGF121 gene transfected hepatocyte was transplanted into the spleen of SD rats. The changes of microvascular density(MVD) counts in the transplanted site and proliferating cell nuclear antigen(PCNA) index of the transplanted hepatocytes were examined.In vitro protein expression of VEGF gene was enough to induce proliferation of human umbilical venous endothelial cells. In vivo VEGF gene transfected hepatocytes could form mass colonization of transplanted hepatocytes and reconstituted liver tissue. Difference of MVD counts in the transplanted site on all groups were not significant. P>0.05; PCNA index of pcDN-A3 VEGF121 gene transfection group increased significantly compared with pcDNA3 control group and no transfection group, P<0.01. PCNA index were 13.13 +/- 2.75, 4.75 +/- 1.58, and 4.63 +/- 1.41, suggesting that in vivo many factors affect the tissue vascularization, and expression of VEGF gene can promote not only proliferation of transplanted hepatocyte but formation of reconstituted liver tissue.VEGF ex vivo gene transfection is an effective way to induce reconstitution of transplanted liver tissue." @default.
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- W2461487989 date "2001-06-01" @default.
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- W2461487989 title "[Effect of vascular endothelial diphosphate glucuronosyltransferase mRNA regulated by alcohol]." @default.
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