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- W2461895236 abstract "ABSTRACT Bacterial multidrug resistance (MDR) efflux pumps are an important mechanism of antibiotic resistance and are required for many pathogens to cause infection. They are also being harnessed to improve microbial biotechnological processes, including biofuel production. Therefore, scientists of many specialties must be able to accurately measure efflux activity. However, myriad methodologies have been described and the most appropriate method is not always clear. Within the scientific literature, many methods are misused or data arising are misinterpreted. The methods for measuring efflux activity can be split into two groups, (i) those that directly measure efflux and (ii) those that measure the intracellular accumulation of a substrate, which is then used to infer efflux activity. Here, we review the methods for measuring efflux and explore the most recent advances in this field, including single-cell or cell-free technologies and mass spectrometry, that are being used to provide more detailed information about efflux pump activity." @default.
- W2461895236 created "2016-07-22" @default.
- W2461895236 creator A5019987484 @default.
- W2461895236 creator A5091502422 @default.
- W2461895236 date "2016-09-07" @default.
- W2461895236 modified "2023-10-16" @default.
- W2461895236 title "How to Measure Export via Bacterial Multidrug Resistance Efflux Pumps" @default.
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- W2461895236 doi "https://doi.org/10.1128/mbio.00840-16" @default.
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