FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2461931611> ?p ?o ?g. }

• W2461931611 abstract "This thesis covers two topics related to transcript elongation in Saccharomyces cerevisiae - the regulation of the phosphatase Fcp1 and transcription-coupled DNA damage repair. Formation of RNA polymerase II (RNAPII) complexes throughout the transcription cycle is mediated in part by the phosphorylation state of the C-terminal domain (CTD) of the largest RNAPII subunit. Although a multitude of kinases can phosphorylate the CTD, currently only one CTD-specific phosphatase, Fcp1, has been identified. This work studies the possibility that Fcp1 might be associated with the elongating form of RNAPII. The phosphatase co-fractionates with RNAPII in association with the elongation factor Elongator. Furthermore, genetic studies show that a double mutant that carries a deletion of an Elongator gene as well as a temperature sensitive fcp1 mutation has a synthetic lethal phenotype at the permissive temperature. In vitro assays using crude extracts demonstrate that the CTD of RNAPII becomes dephosphorylated in a Fcp1-dependent manner. In contrast, in a reconstituted DNA-RNA-RNAPII system, the addition of the purified phosphatase does not stimulate such dephosphoryation. These results indicate a close relationship between Fcp1 phosphatase and the elongating form of RNAPII. Transcription-coupled DNA damage repair is a term applied to the preferential repair of DNA damage on the coding strand within active genes. The second half of this thesis describes the characterisation of nucleotide excision repair (NER) of the intrastrand 1,3-(pGpTpG)-cisplatin lesion in Saccharomyces cerevisiae as well as an attempt to reconstitute a transcription-coupled NER reaction (TC-NER) in vitro. Using modified yeast extracts, the excision products of the above lesion by NER were found to be between 23 and 26 nucleotides long, via incisions around the 15th phosphodiester bond 3' and 7th the phosphodiester bond 5' of the damage. The attempt to reconstitute TC-NER in vitro was hindered by difficulties with the transcription substrate and the functional instability of purified NER proteins." @default.
• W2461931611 created "2016-07-22" @default.
• W2461931611 creator A5045739227 @default.
• W2461931611 date "2001-01-01" @default.
• W2461931611 modified "2023-09-23" @default.
• W2461931611 title "RNA polymerase II during transcript elongation : dealing with DNA damage and staying phosphorylated" @default.
• W2461931611 cites W1491876634 @default.
• W2461931611 cites W1497435365 @default.
• W2461931611 cites W1505354077 @default.
• W2461931611 cites W1522516056 @default.
• W2461931611 cites W1532893184 @default.
• W2461931611 cites W1538598346 @default.
• W2461931611 cites W1546294341 @default.
• W2461931611 cites W1554851690 @default.
• W2461931611 cites W1573178571 @default.
• W2461931611 cites W1577893399 @default.
• W2461931611 cites W1602727746 @default.
• W2461931611 cites W1603620080 @default.
• W2461931611 cites W1609966422 @default.
• W2461931611 cites W1617256403 @default.
• W2461931611 cites W1673271895 @default.
• W2461931611 cites W1886428821 @default.
• W2461931611 cites W1929946817 @default.
• W2461931611 cites W1954289976 @default.
• W2461931611 cites W1965591721 @default.
• W2461931611 cites W1967667723 @default.
• W2461931611 cites W1975494947 @default.
• W2461931611 cites W1976220823 @default.
• W2461931611 cites W1976425824 @default.
• W2461931611 cites W1977598504 @default.
• W2461931611 cites W1978793429 @default.
• W2461931611 cites W1981365624 @default.
• W2461931611 cites W1981900473 @default.
• W2461931611 cites W1984353960 @default.
• W2461931611 cites W1986026506 @default.
• W2461931611 cites W1986144447 @default.
• W2461931611 cites W1987430471 @default.
• W2461931611 cites W1988313137 @default.
• W2461931611 cites W1990493392 @default.
• W2461931611 cites W1991985869 @default.
• W2461931611 cites W1994699072 @default.
• W2461931611 cites W1997974981 @default.
• W2461931611 cites W1998222451 @default.
• W2461931611 cites W2001406822 @default.
• W2461931611 cites W2002806722 @default.
• W2461931611 cites W2003217899 @default.
• W2461931611 cites W2003391433 @default.
• W2461931611 cites W2004041963 @default.
• W2461931611 cites W2004660918 @default.
• W2461931611 cites W2004828705 @default.
• W2461931611 cites W2004837297 @default.
• W2461931611 cites W2005744558 @default.
• W2461931611 cites W2009536322 @default.
• W2461931611 cites W2010049199 @default.
• W2461931611 cites W2013230822 @default.
• W2461931611 cites W2018447274 @default.
• W2461931611 cites W2019429295 @default.
• W2461931611 cites W2022561772 @default.
• W2461931611 cites W2023453929 @default.
• W2461931611 cites W2023735321 @default.
• W2461931611 cites W2025379444 @default.
• W2461931611 cites W2025909991 @default.
• W2461931611 cites W2028805078 @default.
• W2461931611 cites W2030180039 @default.
• W2461931611 cites W2035718056 @default.
• W2461931611 cites W2036552189 @default.
• W2461931611 cites W2038038813 @default.
• W2461931611 cites W2039068784 @default.
• W2461931611 cites W2040779655 @default.
• W2461931611 cites W2042722733 @default.
• W2461931611 cites W2045766250 @default.
• W2461931611 cites W2051031816 @default.
• W2461931611 cites W2053274866 @default.
• W2461931611 cites W2054084236 @default.
• W2461931611 cites W2054212919 @default.
• W2461931611 cites W2056482513 @default.
• W2461931611 cites W2056762049 @default.
• W2461931611 cites W2057243520 @default.
• W2461931611 cites W2059901912 @default.
• W2461931611 cites W2060899717 @default.
• W2461931611 cites W2062582450 @default.
• W2461931611 cites W2067574026 @default.
• W2461931611 cites W2068507797 @default.
• W2461931611 cites W2072836045 @default.
• W2461931611 cites W2073131665 @default.
• W2461931611 cites W2077844248 @default.
• W2461931611 cites W2078578982 @default.
• W2461931611 cites W2079212338 @default.
• W2461931611 cites W2081322742 @default.
• W2461931611 cites W2083854144 @default.
• W2461931611 cites W2084374334 @default.
• W2461931611 cites W2087732988 @default.
• W2461931611 cites W2088163601 @default.
• W2461931611 cites W2096917096 @default.
• W2461931611 cites W2098711782 @default.
• W2461931611 cites W2100649212 @default.
• W2461931611 cites W2100837269 @default.
• W2461931611 cites W2101424708 @default.
• W2461931611 cites W2102514436 @default.
• W2461931611 cites W2103626942 @default.

• next