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- W2462203722 abstract "Background: To determine the pathway via which lipopolysaccharide (LPS) compromises the intestinal epithelial barrier function. Materials and Methods: The human mast cell line HMC-1 cells were stimulated with LPS. The tryptase levels were evaluated in the supernatant. The human colonic epithelial cell line T84 cells were cultured with the supernatant from the culture of the HMC-1 cells that have been activated by LPS. The T84 monolayers' barrier function was evaluated by recording transepithelial electric resistance (TER) and horseradish peroxidase (HRP) flux. Results: HMC-1 cells expressed the toll like receptor 4. LPS stimulation induced HMC-1 cells to release tryptase. The supernatant caused significantly decrease in TER and increase in HRP flux that could be inhibited by pretreatment with tryptase inhibitor BABIM. Conclusion: LPS can induce human mast cells to release chemical mediators such as tryptase that can compromise the intestinal epithelial barrier function." @default.
- W2462203722 created "2016-07-22" @default.
- W2462203722 date "2005-01-01" @default.
- W2462203722 modified "2023-10-14" @default.
- W2462203722 title "Lipopolysaccharide Activates Human Mast Cells To Induce Intestinal Epithelial Barrier Dysfunction" @default.
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- W2462203722 doi "https://doi.org/10.5580/1e5" @default.
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