Matches in SemOpenAlex for { <https://semopenalex.org/work/W2462715084> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2462715084 abstract "Polydimethylsiloxane (PDMS) is the most commonly used material in the manufacture of customized cell culture devices. While there is concern that uncured PDMS oligomers may leach into culture medium and/or hydrophobic molecules may be absorbed into PDMS structures, there is no consensus on how or if PDMS influences cell behaviour. We observed that human umbilical cord blood (CB)-derived CD34+ cells expanded in standard culture medium on PDMS exhibit reduced CD38 surface expression, relative to cells cultured on tissue culture polystyrene (TCP). All-trans retinoic acid (ATRA) induces CD38 expression, and we reasoned that this hydrophobic molecule might be absorbed by PDMS. Through a series of experiments we demonstrated that ATRA-mediated CD38 expression was attenuated when cultures were maintained on PDMS. Medium pre-incubated on PDMS for extended durations resulted in a time-dependant reduction of ATRA in the medium and increasingly attenuated CD38 expression. This indicated a time-dependent absorption of ATRA into the PDMS. To better understand how PDMS might generally influence cell behaviour, Ingenuity Pathway Analysis (IPA) was used to identify potential upstream regulators. This analysis was performed for differentially expressed genes in primary cells including CD34+ haematopoietic progenitor cells, mesenchymal stromal cells (MSC), and keratinocytes, and cell lines including prostate cancer epithelial cells (LNCaP), breast cancer epithelial cells (MCF-7), and myeloid leukaemia cells (KG1a). IPA predicted that the most likely common upstream regulator of perturbed pathways was ATRA. We demonstrate here that ATRA is absorbed by PDMS in a time-dependent manner and results in the concomitant reduced expression of CD38 on the cell surface of CB-derived CD34+ cells." @default.
- W2462715084 created "2016-07-22" @default.
- W2462715084 creator A5015749452 @default.
- W2462715084 creator A5046535725 @default.
- W2462715084 creator A5048942155 @default.
- W2462715084 creator A5052018750 @default.
- W2462715084 creator A5069095946 @default.
- W2462715084 creator A5078738730 @default.
- W2462715084 creator A5086756642 @default.
- W2462715084 date "2016-03-15" @default.
- W2462715084 modified "2023-10-12" @default.
- W2462715084 title "Polydimethylsiloxane (PDMS) modulates CD38 expression, absorbs retinoic acid and may perturb retinoid signalling" @default.
- W2462715084 hasPublicationYear "2016" @default.
- W2462715084 type Work @default.
- W2462715084 sameAs 2462715084 @default.
- W2462715084 citedByCount "0" @default.
- W2462715084 crossrefType "journal-article" @default.
- W2462715084 hasAuthorship W2462715084A5015749452 @default.
- W2462715084 hasAuthorship W2462715084A5046535725 @default.
- W2462715084 hasAuthorship W2462715084A5048942155 @default.
- W2462715084 hasAuthorship W2462715084A5052018750 @default.
- W2462715084 hasAuthorship W2462715084A5069095946 @default.
- W2462715084 hasAuthorship W2462715084A5078738730 @default.
- W2462715084 hasAuthorship W2462715084A5086756642 @default.
- W2462715084 hasConcept C121608353 @default.
- W2462715084 hasConcept C178790620 @default.
- W2462715084 hasConcept C185592680 @default.
- W2462715084 hasConcept C189165786 @default.
- W2462715084 hasConcept C2777699602 @default.
- W2462715084 hasConcept C2779849746 @default.
- W2462715084 hasConcept C2781121885 @default.
- W2462715084 hasConcept C54355233 @default.
- W2462715084 hasConcept C81885089 @default.
- W2462715084 hasConcept C86803240 @default.
- W2462715084 hasConcept C95444343 @default.
- W2462715084 hasConcept C96232424 @default.
- W2462715084 hasConceptScore W2462715084C121608353 @default.
- W2462715084 hasConceptScore W2462715084C178790620 @default.
- W2462715084 hasConceptScore W2462715084C185592680 @default.
- W2462715084 hasConceptScore W2462715084C189165786 @default.
- W2462715084 hasConceptScore W2462715084C2777699602 @default.
- W2462715084 hasConceptScore W2462715084C2779849746 @default.
- W2462715084 hasConceptScore W2462715084C2781121885 @default.
- W2462715084 hasConceptScore W2462715084C54355233 @default.
- W2462715084 hasConceptScore W2462715084C81885089 @default.
- W2462715084 hasConceptScore W2462715084C86803240 @default.
- W2462715084 hasConceptScore W2462715084C95444343 @default.
- W2462715084 hasConceptScore W2462715084C96232424 @default.
- W2462715084 hasOpenAccess W2462715084 @default.
- W2462715084 hasRelatedWork W1787333704 @default.
- W2462715084 hasRelatedWork W1978196503 @default.
- W2462715084 hasRelatedWork W1979885825 @default.
- W2462715084 hasRelatedWork W1987161752 @default.
- W2462715084 hasRelatedWork W2009405081 @default.
- W2462715084 hasRelatedWork W2054592738 @default.
- W2462715084 hasRelatedWork W2060073630 @default.
- W2462715084 hasRelatedWork W2072025654 @default.
- W2462715084 hasRelatedWork W2078707417 @default.
- W2462715084 hasRelatedWork W2095430842 @default.
- W2462715084 hasRelatedWork W2099423350 @default.
- W2462715084 hasRelatedWork W2146173072 @default.
- W2462715084 hasRelatedWork W2297967626 @default.
- W2462715084 hasRelatedWork W2558466867 @default.
- W2462715084 hasRelatedWork W2964354578 @default.
- W2462715084 hasRelatedWork W2965688564 @default.
- W2462715084 hasRelatedWork W2990484275 @default.
- W2462715084 hasRelatedWork W3157119356 @default.
- W2462715084 hasRelatedWork W3171309128 @default.
- W2462715084 hasRelatedWork W2199492835 @default.
- W2462715084 isParatext "false" @default.
- W2462715084 isRetracted "false" @default.
- W2462715084 magId "2462715084" @default.
- W2462715084 workType "article" @default.