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• W2462904652 abstract "We hypothesized that low concentrations of H2O2 could be generated through the electrochemical conversion of oxygen by applying an electric potential to a conductive scaffold and produce a low, but constant, concentration of H2O2 that would be sufficient to destroy biofilms. To test our hypothesis we used a multidrug-resistant Acinetobacter baumannii strain, because this species is often implicated in difficult-to-treat biofilm infections. We used conductive carbon fabric as the scaffold material (e-scaffold). In vitro experiments demonstrated the production of a maximum constant concentration of ~25 μM H2O2 near the e-scaffold surface. An e-scaffold was overlaid onto an existing A. baumannii biofilm, and within 24 h there was a ~4-log reduction in viable bacteria with an ~80% decrease in biofilm surface coverage. A similar procedure was used to overlay an e-scaffold onto an existing A. baumannii biofilm that was grown on a porcine explant. After 24 h, there was a ~3-log reduction in viable bacteria from the infected porcine explants with no observable damage to the underlying mammalian tissue based on a viability assay and histology. This research establishes a novel foundation for an alternative antibiotic-free wound dressing to eliminate biofilms." @default.
• W2462904652 created "2016-07-22" @default.
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• W2462904652 date "2015-10-14" @default.
• W2462904652 modified "2023-10-14" @default.
• W2462904652 title "Electrochemical scaffold generates localized, low concentration of hydrogen peroxide that inhibits bacterial pathogens and biofilms" @default.
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• W2462904652 doi "https://doi.org/10.1038/srep14908" @default.
• W2462904652 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4604468" @default.
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• W2462904652 hasPublicationYear "2015" @default.
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