Matches in SemOpenAlex for { <https://semopenalex.org/work/W2463941344> ?p ?o ?g. }
- W2463941344 endingPage "490" @default.
- W2463941344 startingPage "482" @default.
- W2463941344 abstract "Polymyxa graminis, a root endoparasite of several cereal species, is considered to be non-pathogenic but serves as a vector of various plant viruses belonging to the genera Bymovirus, Furovirus, and Pecluvirus. Specifically, it reduces barley productivity by transmitting the Barley Yellow Mosaic Virus (BaYMV). To date, due to its obligate biotrophic property, no artificial culturing of P. graminis was reported and its quantification was also technically challenging. Here, we developed a novel and simple method to infect P. graminis within sterile barley roots in contamination free by preparing nearly pure zoospore inoculum. Such artificial maintenance of P. graminis was verified based on the presence of various developmental stages in infected barley roots under microscope. In addition, the population of resting spores in host tissue was determined by establishing standard curve between manually counted number of spores and Ct values of 18S rDNA amplification using quantitative real-time PCR. Furthermore, it was validated that standard curve generated was also applicable to estimate the abundance of P. graminis in soil environments. In conclusion, the present study would help to generate a system to investigate the etiological causes as well as management of plant diseases caused by P. graminis and BaYMV in tissue and soil." @default.
- W2463941344 created "2016-07-22" @default.
- W2463941344 creator A5032493354 @default.
- W2463941344 creator A5036667572 @default.
- W2463941344 creator A5051541970 @default.
- W2463941344 creator A5059189060 @default.
- W2463941344 creator A5060058142 @default.
- W2463941344 creator A5060563217 @default.
- W2463941344 creator A5065688046 @default.
- W2463941344 date "2016-07-01" @default.
- W2463941344 modified "2023-09-28" @default.
- W2463941344 title "The Development of Simple Methods for the Maintenance and Quantification of Polymyxa graminis" @default.
- W2463941344 cites W1605577539 @default.
- W2463941344 cites W167654614 @default.
- W2463941344 cites W1752613450 @default.
- W2463941344 cites W1965131931 @default.
- W2463941344 cites W1985242622 @default.
- W2463941344 cites W1988230477 @default.
- W2463941344 cites W1991688574 @default.
- W2463941344 cites W2006049358 @default.
- W2463941344 cites W2006779609 @default.
- W2463941344 cites W2013094737 @default.
- W2463941344 cites W2013785443 @default.
- W2463941344 cites W2018914097 @default.
- W2463941344 cites W2020930922 @default.
- W2463941344 cites W2041173210 @default.
- W2463941344 cites W2060029624 @default.
- W2463941344 cites W2061430940 @default.
- W2463941344 cites W2065543827 @default.
- W2463941344 cites W2094848767 @default.
- W2463941344 cites W2108531551 @default.
- W2463941344 cites W2111102243 @default.
- W2463941344 cites W2113117692 @default.
- W2463941344 cites W2122166988 @default.
- W2463941344 cites W2123483096 @default.
- W2463941344 cites W2127112036 @default.
- W2463941344 cites W2155295587 @default.
- W2463941344 cites W2280276423 @default.
- W2463941344 cites W2313679742 @default.
- W2463941344 cites W2323930444 @default.
- W2463941344 cites W2993563878 @default.
- W2463941344 cites W4245918819 @default.
- W2463941344 doi "https://doi.org/10.1007/s12088-016-0608-2" @default.
- W2463941344 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5061699" @default.
- W2463941344 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27784946" @default.
- W2463941344 hasPublicationYear "2016" @default.
- W2463941344 type Work @default.
- W2463941344 sameAs 2463941344 @default.
- W2463941344 citedByCount "4" @default.
- W2463941344 countsByYear W24639413442019 @default.
- W2463941344 countsByYear W24639413442020 @default.
- W2463941344 crossrefType "journal-article" @default.
- W2463941344 hasAuthorship W2463941344A5032493354 @default.
- W2463941344 hasAuthorship W2463941344A5036667572 @default.
- W2463941344 hasAuthorship W2463941344A5051541970 @default.
- W2463941344 hasAuthorship W2463941344A5059189060 @default.
- W2463941344 hasAuthorship W2463941344A5060058142 @default.
- W2463941344 hasAuthorship W2463941344A5060563217 @default.
- W2463941344 hasAuthorship W2463941344A5065688046 @default.
- W2463941344 hasBestOaLocation W24639413442 @default.
- W2463941344 hasConcept C113735248 @default.
- W2463941344 hasConcept C126831891 @default.
- W2463941344 hasConcept C144024400 @default.
- W2463941344 hasConcept C149923435 @default.
- W2463941344 hasConcept C174618031 @default.
- W2463941344 hasConcept C18903297 @default.
- W2463941344 hasConcept C24990904 @default.
- W2463941344 hasConcept C2908647359 @default.
- W2463941344 hasConcept C59822182 @default.
- W2463941344 hasConcept C86803240 @default.
- W2463941344 hasConcept C99498324 @default.
- W2463941344 hasConceptScore W2463941344C113735248 @default.
- W2463941344 hasConceptScore W2463941344C126831891 @default.
- W2463941344 hasConceptScore W2463941344C144024400 @default.
- W2463941344 hasConceptScore W2463941344C149923435 @default.
- W2463941344 hasConceptScore W2463941344C174618031 @default.
- W2463941344 hasConceptScore W2463941344C18903297 @default.
- W2463941344 hasConceptScore W2463941344C24990904 @default.
- W2463941344 hasConceptScore W2463941344C2908647359 @default.
- W2463941344 hasConceptScore W2463941344C59822182 @default.
- W2463941344 hasConceptScore W2463941344C86803240 @default.
- W2463941344 hasConceptScore W2463941344C99498324 @default.
- W2463941344 hasIssue "4" @default.
- W2463941344 hasLocation W24639413441 @default.
- W2463941344 hasLocation W24639413442 @default.
- W2463941344 hasLocation W24639413443 @default.
- W2463941344 hasLocation W24639413444 @default.
- W2463941344 hasOpenAccess W2463941344 @default.
- W2463941344 hasPrimaryLocation W24639413441 @default.
- W2463941344 hasRelatedWork W1736762500 @default.
- W2463941344 hasRelatedWork W1966524327 @default.
- W2463941344 hasRelatedWork W1983174689 @default.
- W2463941344 hasRelatedWork W1986520063 @default.
- W2463941344 hasRelatedWork W2001679419 @default.
- W2463941344 hasRelatedWork W2053165979 @default.
- W2463941344 hasRelatedWork W2100057771 @default.
- W2463941344 hasRelatedWork W2284383080 @default.
- W2463941344 hasRelatedWork W2774135145 @default.