Matches in SemOpenAlex for { <https://semopenalex.org/work/W2463949013> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2463949013 abstract "Laser capture microdissection (LCM) is a powerful tool for procuring near-pure populations of targeted cell types from specific microscopic regions of tissue sections, by overcoming problems due to tissue heterogeneity and minimizing intermixture and contamination by other cell types. The combination of LCM with various proteomic technologies has enabled high-throughput molecular analysis of human tumors, and provided critical tools in the search for novel disease markers and therapeutic targets. As an example, we describe the application of LCM in dissecting the tumor cells in breast cancer for macromolecular extraction and subsequent protein separation by 2-dimensional gel electrophoresis (2-D GE). The protocols and the key issues involved in preparing ethanol-fixed paraffin-embedded tissue blocks and microscopic sections, microdissecting the cells of interest using the PixCell II LCM system, extracting and separating the cellular proteins by 2-D GE, and preparing selective proteins for peptide mass analysis by mass spectrometry, are discussed. The aim is to provide a practical guide in performing high-throughput microdissection of target cells and gel-based proteomics, which can be adapted to research in cancer formation and growth." @default.
- W2463949013 created "2016-07-22" @default.
- W2463949013 creator A5000546435 @default.
- W2463949013 creator A5023665583 @default.
- W2463949013 date "2008-01-01" @default.
- W2463949013 modified "2023-10-17" @default.
- W2463949013 title "Analysis of Laser Capture Microdissected Cells by 2-Dimensional Gel Electrophoresis" @default.
- W2463949013 cites W1972462568 @default.
- W2463949013 cites W1981308439 @default.
- W2463949013 cites W2014322220 @default.
- W2463949013 cites W2032604597 @default.
- W2463949013 cites W2044577140 @default.
- W2463949013 cites W2092378183 @default.
- W2463949013 cites W2098619516 @default.
- W2463949013 cites W2103012318 @default.
- W2463949013 cites W2122338290 @default.
- W2463949013 cites W2138445021 @default.
- W2463949013 cites W2168474334 @default.
- W2463949013 doi "https://doi.org/10.1007/978-1-59745-117-8_5" @default.
- W2463949013 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18287769" @default.
- W2463949013 hasPublicationYear "2008" @default.
- W2463949013 type Work @default.
- W2463949013 sameAs 2463949013 @default.
- W2463949013 citedByCount "10" @default.
- W2463949013 countsByYear W24639490132012 @default.
- W2463949013 countsByYear W24639490132014 @default.
- W2463949013 crossrefType "book-chapter" @default.
- W2463949013 hasAuthorship W2463949013A5000546435 @default.
- W2463949013 hasAuthorship W2463949013A5023665583 @default.
- W2463949013 hasConcept C104317684 @default.
- W2463949013 hasConcept C104397665 @default.
- W2463949013 hasConcept C11242284 @default.
- W2463949013 hasConcept C116084860 @default.
- W2463949013 hasConcept C121608353 @default.
- W2463949013 hasConcept C125965507 @default.
- W2463949013 hasConcept C150194340 @default.
- W2463949013 hasConcept C153911025 @default.
- W2463949013 hasConcept C185592680 @default.
- W2463949013 hasConcept C46111723 @default.
- W2463949013 hasConcept C54355233 @default.
- W2463949013 hasConcept C55493867 @default.
- W2463949013 hasConcept C70721500 @default.
- W2463949013 hasConcept C86803240 @default.
- W2463949013 hasConcept C96232424 @default.
- W2463949013 hasConceptScore W2463949013C104317684 @default.
- W2463949013 hasConceptScore W2463949013C104397665 @default.
- W2463949013 hasConceptScore W2463949013C11242284 @default.
- W2463949013 hasConceptScore W2463949013C116084860 @default.
- W2463949013 hasConceptScore W2463949013C121608353 @default.
- W2463949013 hasConceptScore W2463949013C125965507 @default.
- W2463949013 hasConceptScore W2463949013C150194340 @default.
- W2463949013 hasConceptScore W2463949013C153911025 @default.
- W2463949013 hasConceptScore W2463949013C185592680 @default.
- W2463949013 hasConceptScore W2463949013C46111723 @default.
- W2463949013 hasConceptScore W2463949013C54355233 @default.
- W2463949013 hasConceptScore W2463949013C55493867 @default.
- W2463949013 hasConceptScore W2463949013C70721500 @default.
- W2463949013 hasConceptScore W2463949013C86803240 @default.
- W2463949013 hasConceptScore W2463949013C96232424 @default.
- W2463949013 hasLocation W24639490131 @default.
- W2463949013 hasLocation W24639490132 @default.
- W2463949013 hasOpenAccess W2463949013 @default.
- W2463949013 hasPrimaryLocation W24639490131 @default.
- W2463949013 hasRelatedWork W1553919822 @default.
- W2463949013 hasRelatedWork W1970434563 @default.
- W2463949013 hasRelatedWork W2005833192 @default.
- W2463949013 hasRelatedWork W2006373898 @default.
- W2463949013 hasRelatedWork W2028440758 @default.
- W2463949013 hasRelatedWork W2053583931 @default.
- W2463949013 hasRelatedWork W2065673022 @default.
- W2463949013 hasRelatedWork W2138445021 @default.
- W2463949013 hasRelatedWork W2161768473 @default.
- W2463949013 hasRelatedWork W6306124 @default.
- W2463949013 isParatext "false" @default.
- W2463949013 isRetracted "false" @default.
- W2463949013 magId "2463949013" @default.
- W2463949013 workType "book-chapter" @default.