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- W2463968965 abstract "This chapter describes methods employ in the research on the biochemistry of sperm–egg interaction. These methods devised for this are handling, labeling, and fractionating sea urchin spermatozoa. A pure sperm suspension can be prepared comprising composition of semen and removal of pigmented phagocytes and with method for washing sperm. Heavy metal chelators, amino acids, and peptides prolong life of sperm. A simple method for determining sperm concentration is to measure the turbidity of a sperm suspension with a spectrophotometer and relating the value to a standard curve constructed by hemocytometer counts. The sperm components are labeled by radioiodination of membrane glycoproteins, labeling with [125I]diiodofluorescein isothiocyanate (IFC), labeling the surface with lectins and antibodies, and labeling sperm with radiophosphate. The isolation of sperm components comprises separation of head from flagellum, isolation of components of the sperm head, and isolation of the plasma membrane." @default.
- W2463968965 created "2016-07-22" @default.
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- W2463968965 date "1986-01-01" @default.
- W2463968965 modified "2023-10-14" @default.
- W2463968965 title "Chapter 2 Handling, Labeling, and Fractionating Sea Urchin Spermatozoa" @default.
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- W2463968965 doi "https://doi.org/10.1016/s0091-679x(08)60340-4" @default.
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