FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2464272022> ?p ?o ?g. }

• W2464272022 abstract "Objective: Streptococcus mitis is a predominant oral colonizer, but difficulties in genetic manipulation of this species have hampered our understanding of the mechanisms it uses for colonization of oral surfaces. The aim of this study was to reveal optimal conditions for natural genetic transformation in S. mitis and illustrate its application in direct genome editing. Methods: Luciferase reporter assays were used to assess gene expression of the alternative sigma factor (σX) in combination with natural transformation experiments to evaluate the efficiency by which S. mitis activates the competence system and incorporates exogenous DNA. Optimal amounts and sources of donor DNA (chromosomal, amplicon, or replicative plasmid), concentrations of synthetic competence-stimulating peptide, and transformation media were assessed. Results: A semi-defined medium showed much improved results for response to the competence stimulating peptide (CSP) when compared to rich media. The use of a donor amplicon with large homology flanking regions also provided higher transformation rates. Overall, an increase of transformation efficiencies from 0.001% or less to over 30% was achieved with the developed protocol. We further describe the construction of a markerless mutant based on this high efficiency strategy. Conclusions: We optimized competence development in S. mitis, by use of semi-defined medium and appropriate concentrations of synthetic competence factor. Combined with the use of a large amplicon of donor DNA, this method allowed easy and direct editing of the S. mitis genome, broadening the spectrum of possible downstream applications of natural transformation in this species." @default.
• W2464272022 created "2016-07-22" @default.
• W2464272022 creator A5012397786 @default.
• W2464272022 creator A5031247653 @default.
• W2464272022 creator A5045588156 @default.
• W2464272022 creator A5091270805 @default.
• W2464272022 date "2016-07-05" @default.
• W2464272022 modified "2023-09-27" @default.
• W2464272022 title "Overcoming the Barrier of Low Efficiency during Genetic Transformation of Streptococcus mitis" @default.
• W2464272022 cites W1518272160 @default.
• W2464272022 cites W1538069795 @default.
• W2464272022 cites W1971661577 @default.
• W2464272022 cites W1977782657 @default.
• W2464272022 cites W1998306938 @default.
• W2464272022 cites W2002020281 @default.
• W2464272022 cites W2011939723 @default.
• W2464272022 cites W2015616512 @default.
• W2464272022 cites W2018366249 @default.
• W2464272022 cites W2021354930 @default.
• W2464272022 cites W2029047684 @default.
• W2464272022 cites W2031210646 @default.
• W2464272022 cites W2036528751 @default.
• W2464272022 cites W2037876790 @default.
• W2464272022 cites W2049344804 @default.
• W2464272022 cites W2069600367 @default.
• W2464272022 cites W2074726414 @default.
• W2464272022 cites W2076642197 @default.
• W2464272022 cites W2079630246 @default.
• W2464272022 cites W2080263535 @default.
• W2464272022 cites W2080470002 @default.
• W2464272022 cites W2094054930 @default.
• W2464272022 cites W2095048146 @default.
• W2464272022 cites W2097002929 @default.
• W2464272022 cites W2108281648 @default.
• W2464272022 cites W2114837947 @default.
• W2464272022 cites W2128894039 @default.
• W2464272022 cites W2129527290 @default.
• W2464272022 cites W2135167379 @default.
• W2464272022 cites W2136856303 @default.
• W2464272022 cites W2142596061 @default.
• W2464272022 cites W2148328424 @default.
• W2464272022 cites W2154901892 @default.
• W2464272022 cites W2158605840 @default.
• W2464272022 cites W2159865525 @default.
• W2464272022 cites W2159872412 @default.
• W2464272022 cites W2162366970 @default.
• W2464272022 cites W2165716810 @default.
• W2464272022 cites W2170396343 @default.
• W2464272022 cites W2187659669 @default.
• W2464272022 cites W2193269992 @default.
• W2464272022 cites W2339462686 @default.
• W2464272022 cites W2400667230 @default.
• W2464272022 cites W2795165614 @default.
• W2464272022 doi "https://doi.org/10.3389/fmicb.2016.01009" @default.
• W2464272022 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4932118" @default.
• W2464272022 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27458432" @default.
• W2464272022 hasPublicationYear "2016" @default.
• W2464272022 type Work @default.
• W2464272022 sameAs 2464272022 @default.
• W2464272022 citedByCount "17" @default.
• W2464272022 countsByYear W24642720222017 @default.
• W2464272022 countsByYear W24642720222018 @default.
• W2464272022 countsByYear W24642720222019 @default.
• W2464272022 countsByYear W24642720222020 @default.
• W2464272022 countsByYear W24642720222021 @default.
• W2464272022 countsByYear W24642720222022 @default.
• W2464272022 crossrefType "journal-article" @default.
• W2464272022 hasAuthorship W2464272022A5012397786 @default.
• W2464272022 hasAuthorship W2464272022A5031247653 @default.
• W2464272022 hasAuthorship W2464272022A5045588156 @default.
• W2464272022 hasAuthorship W2464272022A5091270805 @default.
• W2464272022 hasBestOaLocation W24642720221 @default.
• W2464272022 hasConcept C104317684 @default.
• W2464272022 hasConcept C141231307 @default.
• W2464272022 hasConcept C178050479 @default.
• W2464272022 hasConcept C186852380 @default.
• W2464272022 hasConcept C204241405 @default.
• W2464272022 hasConcept C22744801 @default.
• W2464272022 hasConcept C2777480049 @default.
• W2464272022 hasConcept C2778276568 @default.
• W2464272022 hasConcept C2779656422 @default.
• W2464272022 hasConcept C49105822 @default.
• W2464272022 hasConcept C523546767 @default.
• W2464272022 hasConcept C54355233 @default.
• W2464272022 hasConcept C552990157 @default.
• W2464272022 hasConcept C70721500 @default.
• W2464272022 hasConcept C8185291 @default.
• W2464272022 hasConcept C86803240 @default.
• W2464272022 hasConceptScore W2464272022C104317684 @default.
• W2464272022 hasConceptScore W2464272022C141231307 @default.
• W2464272022 hasConceptScore W2464272022C178050479 @default.
• W2464272022 hasConceptScore W2464272022C186852380 @default.
• W2464272022 hasConceptScore W2464272022C204241405 @default.
• W2464272022 hasConceptScore W2464272022C22744801 @default.
• W2464272022 hasConceptScore W2464272022C2777480049 @default.
• W2464272022 hasConceptScore W2464272022C2778276568 @default.
• W2464272022 hasConceptScore W2464272022C2779656422 @default.
• W2464272022 hasConceptScore W2464272022C49105822 @default.
• W2464272022 hasConceptScore W2464272022C523546767 @default.
• W2464272022 hasConceptScore W2464272022C54355233 @default.

• next