Matches in SemOpenAlex for { <https://semopenalex.org/work/W2464321038> ?p ?o ?g. }
- W2464321038 endingPage "172" @default.
- W2464321038 startingPage "172" @default.
- W2464321038 abstract "The bacteriophage lambda replication initiation protein P exhibits a toxic effect on its Escherichia coli (E. coli) host, likely due to the formation of a dead-end P-DnaB complex, sequestering the replicative DnaB helicase from further activity. Intracellular expression of P triggers SOS-independent cellular filamentation and rapidly cures resident ColE1 plasmids. The toxicity of P is suppressed by alleles of P or dnaB. We asked whether P buildup within a cell can influence E. coli replication fidelity. The influence of P expression from a defective prophage, or when cloned and expressed from a plasmid was examined by screening for auxotrophic mutants, or by selection for rifampicin resistant (RifR) cells acquiring mutations within the rpoB gene encoding the β-subunit of RNA polymerase (RNAP), nine of which proved unique. Using fluctuation assays, we show that the intracellular expression of P evokes a mutator effect. Most of the RifR mutants remained PS and localized to the Rif binding pocket in RNAP, but a subset acquired a PR phenotype, lost sensitivity to ColE1 plasmid curing, and localized outside of the pocket. One PR mutation was identical to rpo*Q148P, which alleviates the UV-sensitivity of ruv strains defective in the migration and resolution of Holliday junctions and destabilizes stalled RNAP elongation complexes. The results suggest that P-DnaB sequestration is mutagenic and supports an earlier observation that P can interact with RNAP." @default.
- W2464321038 created "2016-07-22" @default.
- W2464321038 creator A5016143189 @default.
- W2464321038 creator A5034412333 @default.
- W2464321038 creator A5061128899 @default.
- W2464321038 creator A5062600625 @default.
- W2464321038 creator A5076075245 @default.
- W2464321038 creator A5091332739 @default.
- W2464321038 date "2016-06-22" @default.
- W2464321038 modified "2023-09-26" @default.
- W2464321038 title "Lambda gpP-DnaB Helicase Sequestration and gpP-RpoB Associated Effects: On Screens for Auxotrophs, Selection for RifR, Toxicity, Mutagenicity, Plasmid Curing" @default.
- W2464321038 cites W107673453 @default.
- W2464321038 cites W10945592 @default.
- W2464321038 cites W119231150 @default.
- W2464321038 cites W1504527878 @default.
- W2464321038 cites W1507565214 @default.
- W2464321038 cites W1512516275 @default.
- W2464321038 cites W1517396783 @default.
- W2464321038 cites W1537586850 @default.
- W2464321038 cites W1541498669 @default.
- W2464321038 cites W1545066603 @default.
- W2464321038 cites W1552198120 @default.
- W2464321038 cites W1559858723 @default.
- W2464321038 cites W1563195770 @default.
- W2464321038 cites W1567164927 @default.
- W2464321038 cites W1574146385 @default.
- W2464321038 cites W1579482312 @default.
- W2464321038 cites W1580214297 @default.
- W2464321038 cites W1591954229 @default.
- W2464321038 cites W1592750650 @default.
- W2464321038 cites W1603564685 @default.
- W2464321038 cites W1618334441 @default.
- W2464321038 cites W1624476691 @default.
- W2464321038 cites W1866948286 @default.
- W2464321038 cites W1887714095 @default.
- W2464321038 cites W1916074658 @default.
- W2464321038 cites W1968288171 @default.
- W2464321038 cites W1970341643 @default.
- W2464321038 cites W1971803523 @default.
- W2464321038 cites W1972037852 @default.
- W2464321038 cites W1974141130 @default.
- W2464321038 cites W1982618160 @default.
- W2464321038 cites W1992394095 @default.
- W2464321038 cites W1995227830 @default.
- W2464321038 cites W1999054743 @default.
- W2464321038 cites W1999718691 @default.
- W2464321038 cites W2002367826 @default.
- W2464321038 cites W2004392681 @default.
- W2464321038 cites W2004768642 @default.
- W2464321038 cites W2006646718 @default.
- W2464321038 cites W2007671505 @default.
- W2464321038 cites W2024759819 @default.
- W2464321038 cites W2025835178 @default.
- W2464321038 cites W2030903100 @default.
- W2464321038 cites W2033721606 @default.
- W2464321038 cites W2034175604 @default.
- W2464321038 cites W2034840813 @default.
- W2464321038 cites W2035777204 @default.
- W2464321038 cites W2041214497 @default.
- W2464321038 cites W2042511341 @default.
- W2464321038 cites W2048100858 @default.
- W2464321038 cites W2048680704 @default.
- W2464321038 cites W2050325858 @default.
- W2464321038 cites W2053582198 @default.
- W2464321038 cites W2058669696 @default.
- W2464321038 cites W2059754005 @default.
- W2464321038 cites W2060255621 @default.
- W2464321038 cites W2061027931 @default.
- W2464321038 cites W2066179156 @default.
- W2464321038 cites W2068372433 @default.
- W2464321038 cites W2069028813 @default.
- W2464321038 cites W2069131288 @default.
- W2464321038 cites W2070059581 @default.
- W2464321038 cites W2072139304 @default.
- W2464321038 cites W2076147748 @default.
- W2464321038 cites W2078170734 @default.
- W2464321038 cites W2078664051 @default.
- W2464321038 cites W2080159433 @default.
- W2464321038 cites W2088553142 @default.
- W2464321038 cites W2106535326 @default.
- W2464321038 cites W2109428056 @default.
- W2464321038 cites W2117371546 @default.
- W2464321038 cites W2121942815 @default.
- W2464321038 cites W2127462345 @default.
- W2464321038 cites W2129881217 @default.
- W2464321038 cites W2130940913 @default.
- W2464321038 cites W2132208192 @default.
- W2464321038 cites W2135581763 @default.
- W2464321038 cites W2135625588 @default.
- W2464321038 cites W2149691257 @default.
- W2464321038 cites W2150164597 @default.
- W2464321038 cites W2152479833 @default.
- W2464321038 cites W2169220413 @default.
- W2464321038 cites W22874528 @default.
- W2464321038 cites W2306853147 @default.
- W2464321038 cites W2314807009 @default.
- W2464321038 cites W2316845746 @default.
- W2464321038 cites W2316878241 @default.