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• W2464356320 abstract "Olive oil is considered a premium product for its nutritional value and health benefits, and the ability to define its origin and varietal composition is a key step towards ensuring the traceability of the product. However, isolating the DNA from such a matrix is a difficult task. In this study, the quality and quantity of olive oil DNA, isolated using four different DNA isolation protocols, was evaluated using the qRT-PCR and ddPCR techniques. The results indicate that CTAB-based extraction methods were the best for unfiltered oil, while Nucleo Spin-based extraction protocols showed greater overall reproducibility. The use of both qRT-PCR and ddPCR led to the absolute quantification of the DNA copy number. The results clearly demonstrate the importance of the choice of DNA-isolation protocol, which should take into consideration the qualitative aspects of DNA and the evaluation of the amplified DNA copy number." @default.
• W2464356320 created "2016-07-22" @default.
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• W2464356320 date "2016-12-01" @default.
• W2464356320 modified "2023-10-15" @default.
• W2464356320 title "Absolute quantification of olive oil DNA by droplet digital-PCR (ddPCR): Comparison of isolation and amplification methodologies" @default.
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• W2464356320 doi "https://doi.org/10.1016/j.foodchem.2016.06.086" @default.
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• W2464356320 hasPublicationYear "2016" @default.
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