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• W2464433332 abstract "Dendritic cell (DCs) are essential antigen processing and presentation cells that play a key role in the immune response. In this study, DCs were co‑cultured with cytokine‑induced killer cells (DC‑CIKs) in vitro to detect changes in cell proliferation, cell phenotype and cell cytotoxicity. The results revealed that the DCs were suitable for co‑culture with CIKs at day 7, and that cell quantity of DC‑CIKs was lower than that of CIKs until day 11, but it was significantly improved to 1.17‑fold that of CIKs at day 13. Flow cytometry was used to detect the cell phenotype of CIKs and DC‑CIKs. Compared with CIKs at day 13, the percentage of CD3+, CD3+CD4+, CD3+CD8+ and CD3+CD56+ T cells in DC‑CIKs was significantly improved 1.02, 1.79, 1.26 and 2.44‑fold, respectively. In addition, trypan blue staining analysis demonstrated that the cell viability of CIKs and DC‑CIKs was 96% and 98%, respectively. Furthermore, 3‑(4,5‑dimethylthiazol‑2‑yl)-2,5-diphenyltetrazolium bromide (MTT) analysis verified that CIK and DC‑CIK cytotoxicity in Hela cells was 58% and 80%, respectively, with a significant difference. Taken together, our results indicate that the cell proliferation, cell phenotype and antitumor activity of CIKs were all enhanced following co‑culture with DCs in vitro. These results are likely to be useful for DC‑CIK application in antitumor therapies." @default.
• W2464433332 created "2016-07-22" @default.
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• W2464433332 date "2016-07-11" @default.
• W2464433332 modified "2023-10-02" @default.
• W2464433332 title "Influence of autologous dendritic cells on cytokine-induced killer cell proliferation, cell phenotype and antitumor activity in vitro" @default.
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• W2464433332 doi "https://doi.org/10.3892/ol.2016.4839" @default.
• W2464433332 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4998518" @default.
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