Matches in SemOpenAlex for { <https://semopenalex.org/work/W2464694264> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2464694264 abstract "The hallmark of cancer is the ability of cancer cells to disseminate and invade to distant organs. Metastasis accounts for over 90% of cancer related deaths and therefore requires a better understanding of the metastatic cascade to develop better therapeutic regimens. The metastatic process is heavily influenced by the extracellular matrix (ECM) density and composition of the surrounding tumour microenvironment. These microenvironmental cues also regulate the angiogenic processes within a tumour, facilitating the spread of cancer cells. The aim of this thesis was to develop a biomimetic 3D in vitro model of colorectal cancer with controllable matrix parameters that regulate cancer invasion, the formation of a primitive vascular network and response to targeted drug treatment. A novel 3D in vitro cancer model was established based on the removal of interstitial fluid in collagen type I hydrogels. Colorectal cancer cells cultured in dense collagen gels formed well-defined cellular aggregates mimicking avascular micrometastases observed in vivo. Cancer cells invaded from the artificial cancer mass (ACM) into the stromal surround in cell specific patterns, either as spherical aggregates or cell sheets. Invasion into a denser collagen matrix altered the migration pattern predominantly to cell sheets. Laminin was found to enhance the invasion profile of colorectal cancer cells independent of stromal matrix density. The pro-invasive and epithelial-mesenchymal transition (EMT) markers MMP7 and vimentin were upregulated in 3D cultures in comparison to 2D monolayers. Stromal cultures containing fibroblasts and endothelial cells formed highly branched end-to-end vascular networks in the presence of laminin. The addition of cancer cells produces significantly longer but substantially less interconnected vascular networks mimicking in vivo ‘leaky’ tumour vasculature. The loss of CK20 by invading cancer cells significantly correlated with the overall distance of invasion into the stromal surround. Although the expression of the biomarker EGFR was upregulated in 3D, targeted treatment using cetuximab lead to a greater inhibition profile in 2D monolayers than in 3D cultures. Drug resistance in 3D cultures corresponded with the presence of cancer stem cells. These findings signify the importance of 3D in vitro cancer models as important tools to study the effect of microenvironmental conditions on tumour malignancy." @default.
- W2464694264 created "2016-07-22" @default.
- W2464694264 creator A5077850592 @default.
- W2464694264 date "2016-04-28" @default.
- W2464694264 modified "2023-09-28" @default.
- W2464694264 title "Development of a 3D in vitro model of cancer progression" @default.
- W2464694264 hasPublicationYear "2016" @default.
- W2464694264 type Work @default.
- W2464694264 sameAs 2464694264 @default.
- W2464694264 citedByCount "0" @default.
- W2464694264 crossrefType "dissertation" @default.
- W2464694264 hasAuthorship W2464694264A5077850592 @default.
- W2464694264 hasConcept C121608353 @default.
- W2464694264 hasConcept C142724271 @default.
- W2464694264 hasConcept C147447768 @default.
- W2464694264 hasConcept C16930146 @default.
- W2464694264 hasConcept C185592680 @default.
- W2464694264 hasConcept C189165786 @default.
- W2464694264 hasConcept C203014093 @default.
- W2464694264 hasConcept C204232928 @default.
- W2464694264 hasConcept C2776107976 @default.
- W2464694264 hasConcept C2779013556 @default.
- W2464694264 hasConcept C502942594 @default.
- W2464694264 hasConcept C526805850 @default.
- W2464694264 hasConcept C54355233 @default.
- W2464694264 hasConcept C71924100 @default.
- W2464694264 hasConcept C86803240 @default.
- W2464694264 hasConcept C95444343 @default.
- W2464694264 hasConcept C96232424 @default.
- W2464694264 hasConceptScore W2464694264C121608353 @default.
- W2464694264 hasConceptScore W2464694264C142724271 @default.
- W2464694264 hasConceptScore W2464694264C147447768 @default.
- W2464694264 hasConceptScore W2464694264C16930146 @default.
- W2464694264 hasConceptScore W2464694264C185592680 @default.
- W2464694264 hasConceptScore W2464694264C189165786 @default.
- W2464694264 hasConceptScore W2464694264C203014093 @default.
- W2464694264 hasConceptScore W2464694264C204232928 @default.
- W2464694264 hasConceptScore W2464694264C2776107976 @default.
- W2464694264 hasConceptScore W2464694264C2779013556 @default.
- W2464694264 hasConceptScore W2464694264C502942594 @default.
- W2464694264 hasConceptScore W2464694264C526805850 @default.
- W2464694264 hasConceptScore W2464694264C54355233 @default.
- W2464694264 hasConceptScore W2464694264C71924100 @default.
- W2464694264 hasConceptScore W2464694264C86803240 @default.
- W2464694264 hasConceptScore W2464694264C95444343 @default.
- W2464694264 hasConceptScore W2464694264C96232424 @default.
- W2464694264 hasLocation W24646942641 @default.
- W2464694264 hasOpenAccess W2464694264 @default.
- W2464694264 hasPrimaryLocation W24646942641 @default.
- W2464694264 hasRelatedWork W1508051245 @default.
- W2464694264 hasRelatedWork W1559090524 @default.
- W2464694264 hasRelatedWork W1860605136 @default.
- W2464694264 hasRelatedWork W1998164580 @default.
- W2464694264 hasRelatedWork W2037309618 @default.
- W2464694264 hasRelatedWork W2060086044 @default.
- W2464694264 hasRelatedWork W2076306819 @default.
- W2464694264 hasRelatedWork W2108231528 @default.
- W2464694264 hasRelatedWork W2177483881 @default.
- W2464694264 hasRelatedWork W2338943245 @default.
- W2464694264 hasRelatedWork W2372992398 @default.
- W2464694264 hasRelatedWork W2510046850 @default.
- W2464694264 hasRelatedWork W2657389501 @default.
- W2464694264 hasRelatedWork W2739497725 @default.
- W2464694264 hasRelatedWork W2764186557 @default.
- W2464694264 hasRelatedWork W2806840874 @default.
- W2464694264 hasRelatedWork W2990003133 @default.
- W2464694264 hasRelatedWork W3010618749 @default.
- W2464694264 hasRelatedWork W3030443317 @default.
- W2464694264 hasRelatedWork W3044870382 @default.
- W2464694264 isParatext "false" @default.
- W2464694264 isRetracted "false" @default.
- W2464694264 magId "2464694264" @default.
- W2464694264 workType "dissertation" @default.