FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2464885033> ?p ?o ?g. }

• W2464885033 endingPage "196" @default.
• W2464885033 startingPage "187" @default.
• W2464885033 abstract "The potential and benefits of isotope-coded labeling in the context of MS-based glycan profiling are evaluated focusing on the analysis of O-glycans. For this purpose, a derivatization strategy using d0/d5-1-phenyl-3-methyl-5-pyrazolone (PMP) is employed, allowing O-glycan release and derivatization to be achieved in one single step. The paper demonstrates that this release and derivatization reaction can be carried out also in-gel with only marginal loss in sensitivity compared to in-solution derivatization. Such an effective in-gel reaction allows one to extend this release/labeling method also to glycoprotein/glycoform samples pre-separated by gel-electrophoresis without the need of extracting the proteins/digested peptides from the gel. With highly O-glycosylated proteins (e.g. mucins) LODs in the range of 0.4 μg glycoprotein (100 fmol) loaded onto the electrophoresis gel can be attained, with minor glycosylated proteins (like IgAs, FVII, FIX) the LODs were in the range of 80–100 μg (250 pmol–1.5 nmol) glycoprotein loaded onto the gel. As second aspect, the potential of isotope coded labeling as internal standardization strategy for the reliable determination of quantitative glycan profiles via MALDI-MS is investigated. Towards this goal, a number of established and emerging MALDI matrices were tested for PMP-glycan quantitation, and their performance is compared with that of ESI-based measurements. The crystalline matrix 2,6-dihydroxyacetophenone (DHAP) and the ionic liquid matrix N,N-diisopropyl-ethyl-ammonium 2,4,6-trihydroxyacetophenone (DIEA-THAP) showed potential for MALDI-based quantitation of PMP-labeled O-glycans. We also provide a comprehensive overview on the performance of MS-based glycan quantitation approaches by comparing sensitivity, LOD, accuracy and repeatability data obtained with RP-HPLC-ESI-MS, stand-alone nano-ESI-MS with a spray-nozzle chip, and MALDI-MS. Finally, the suitability of the isotope-coded PMP labeling strategy for O-glycan profiling of biological important proteins is demonstrated by comparative analysis of IgA immunoglobulins and two coagulation factors." @default.
• W2464885033 created "2016-07-22" @default.
• W2464885033 creator A5008817899 @default.
• W2464885033 creator A5051581775 @default.
• W2464885033 creator A5070086178 @default.
• W2464885033 date "2016-09-01" @default.
• W2464885033 modified "2023-10-17" @default.
• W2464885033 title "Quantitative profiling of O-glycans by electrospray ionization- and matrix-assisted laser desorption ionization-time-of-flight-mass spectrometry after in-gel derivatization with isotope-coded 1-phenyl-3-methyl-5-pyrazolone" @default.
• W2464885033 cites W1588711507 @default.
• W2464885033 cites W1706543614 @default.
• W2464885033 cites W1968361672 @default.
• W2464885033 cites W1971914313 @default.
• W2464885033 cites W1972934137 @default.
• W2464885033 cites W1973283944 @default.
• W2464885033 cites W1975201713 @default.
• W2464885033 cites W1977112124 @default.
• W2464885033 cites W1992497040 @default.
• W2464885033 cites W1996015876 @default.
• W2464885033 cites W1999629817 @default.
• W2464885033 cites W2000087710 @default.
• W2464885033 cites W2001634746 @default.
• W2464885033 cites W2007496498 @default.
• W2464885033 cites W2014847406 @default.
• W2464885033 cites W2015898359 @default.
• W2464885033 cites W2023692109 @default.
• W2464885033 cites W2028019069 @default.
• W2464885033 cites W2034474150 @default.
• W2464885033 cites W2035788412 @default.
• W2464885033 cites W2040828445 @default.
• W2464885033 cites W2043702418 @default.
• W2464885033 cites W2044658180 @default.
• W2464885033 cites W2046424545 @default.
• W2464885033 cites W2048182696 @default.
• W2464885033 cites W2055400546 @default.
• W2464885033 cites W2055507506 @default.
• W2464885033 cites W2055585414 @default.
• W2464885033 cites W2063011175 @default.
• W2464885033 cites W2063103181 @default.
• W2464885033 cites W2065481451 @default.
• W2464885033 cites W2070810383 @default.
• W2464885033 cites W2071335845 @default.
• W2464885033 cites W2074371856 @default.
• W2464885033 cites W2075210096 @default.
• W2464885033 cites W2077963235 @default.
• W2464885033 cites W2088433975 @default.
• W2464885033 cites W2092193065 @default.
• W2464885033 cites W2099540110 @default.
• W2464885033 cites W2100837269 @default.
• W2464885033 cites W2107428675 @default.
• W2464885033 cites W2109751008 @default.
• W2464885033 cites W2117852760 @default.
• W2464885033 cites W2119918510 @default.
• W2464885033 cites W2121237686 @default.
• W2464885033 cites W2124551362 @default.
• W2464885033 cites W2133076634 @default.
• W2464885033 cites W2135401970 @default.
• W2464885033 cites W2153241884 @default.
• W2464885033 cites W2155628978 @default.
• W2464885033 cites W2335113735 @default.
• W2464885033 cites W801098645 @default.
• W2464885033 doi "https://doi.org/10.1016/j.aca.2016.06.032" @default.
• W2464885033 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27543027" @default.
• W2464885033 hasPublicationYear "2016" @default.
• W2464885033 type Work @default.
• W2464885033 sameAs 2464885033 @default.
• W2464885033 citedByCount "11" @default.
• W2464885033 countsByYear W24648850332016 @default.
• W2464885033 countsByYear W24648850332017 @default.
• W2464885033 countsByYear W24648850332018 @default.
• W2464885033 countsByYear W24648850332019 @default.
• W2464885033 countsByYear W24648850332020 @default.
• W2464885033 countsByYear W24648850332021 @default.
• W2464885033 countsByYear W24648850332022 @default.
• W2464885033 crossrefType "journal-article" @default.
• W2464885033 hasAuthorship W2464885033A5008817899 @default.
• W2464885033 hasAuthorship W2464885033A5051581775 @default.
• W2464885033 hasAuthorship W2464885033A5070086178 @default.
• W2464885033 hasConcept C108625454 @default.
• W2464885033 hasConcept C150394285 @default.
• W2464885033 hasConcept C162356407 @default.
• W2464885033 hasConcept C162711632 @default.
• W2464885033 hasConcept C178790620 @default.
• W2464885033 hasConcept C185592680 @default.
• W2464885033 hasConcept C194451485 @default.
• W2464885033 hasConcept C206212055 @default.
• W2464885033 hasConcept C2776923573 @default.
• W2464885033 hasConcept C40684141 @default.
• W2464885033 hasConcept C43617362 @default.
• W2464885033 hasConcept C55493867 @default.
• W2464885033 hasConcept C75280812 @default.
• W2464885033 hasConceptScore W2464885033C108625454 @default.
• W2464885033 hasConceptScore W2464885033C150394285 @default.
• W2464885033 hasConceptScore W2464885033C162356407 @default.
• W2464885033 hasConceptScore W2464885033C162711632 @default.
• W2464885033 hasConceptScore W2464885033C178790620 @default.
• W2464885033 hasConceptScore W2464885033C185592680 @default.
• W2464885033 hasConceptScore W2464885033C194451485 @default.
• W2464885033 hasConceptScore W2464885033C206212055 @default.

• next