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• W2465375500 abstract "It has been previously shown that Electrical Pulse Stimulation (EPS) simulates exercise in vitro using a cell culture model. Also, it has been demonstrated that human skeletal muscle myotubes cultured in vitro, retain certain in vivo characteristics of the donors. However, it is not clear whether myotubes collected from non-diabetic vs. type 2 diabetes donors respond similarly to the EPS stimulus. PURPOSE: We compared the exercise-induced adaptations in lipid and mitochondrial content after EPS in myotubes collected from sedentary lean, athletes and type 2 diabetes donors. METHODS: EPS was applied to human myotubes under three different durations: acute (bipolar pulses of 100 Hz for 200 ms every 5th second; 30V for 60 min) 24 hr., and 48 hr. (single bipolar pulses of 1 Hz for 2 ms; 30V continuously). Stimulated cells were collected immediately after the end of stimulation (early harvest) and 24 hr. after the end of stimulation (late harvest). Mitochondrial and lipid content were measured by primary antibody for complex IV and bodipy green dye, respectively. Fluoroskan ascent microplate fluorometer was used to quantify fluorescence signals. RESULTS: 24 hr. early harvest EPS resulted in a significantly higher (p<0.05) lipid content in both non-diabetic and T2D myotubes compared to respective unstimulated controls. There was no change in lipid content in both non-diabetic and T2D myotubes after acute, 24 hr. and 48 hr. late harvest EPS. Mitochondrial content in non-diabetic myotubes was significantly higher (p<0.05) compared to respective controls after 24 hr. and 48 hr. of early harvest EPS and significantly lower (p<0.05) after acute late harvest EPS. Myotubes from T2D showed a significantly lower (p<0.05) mitochondrial content after acute and 24 hr early harvest EPS and significantly higher (p<0.05) after 24 hr. late harvest EPS. CONCLUSION: EPS induced increase in mitochondrial content although evident in myotubes from non-diabetic donors, is not evident in myotubes collected from donors with T2D. Furthermore, collection time seems to be a key factor affecting lipid and mitochondrial content in both myotubes from non-diabetic and type 2 diabetic subjects." @default.
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• W2465375500 date "2015-05-01" @default.
• W2465375500 modified "2023-10-14" @default.
• W2465375500 title "Electrical Pulse Stimulation Increases Mitochondrial Content In Lean, But Not In Type 2 Diabetic Myotubes" @default.
• W2465375500 doi "https://doi.org/10.1249/01.mss.0000466014.44604.d1" @default.
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