FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2465461191> ?p ?o ?g. }

• W2465461191 abstract "Thesis by publication.%%%%%%%%Bibliography: p. 184-211.%%%%Introduction  – Materials and methods  – Isolation and identification of fungi from koala faeces and screening for their enzyme activities of biotechnological interest  – Cultivation of selected fungal isolates from koala faeces in liquid media for further enzyme characterisation  – The secretome of Doratomyces stemonitis C8, isolated from koala faeces  – Main findings and future prospects.%%%%Filamentous fungi secrete enzymes to break down complex substances in the environment into smaller molecules they can use for nutrition. Investigation of the enzymes secreted by a fungus can lead to a better understanding of how it survives in a natural habitat; furthermore, new enzymes with potential for industrial applications can be revealed. In this work, the enzyme activities of fungi from koala faeces were investigated. As a result of a diet of Eucalyptus leaves, koala faeces are composed of recalcitrant plant cell wall polymers (cellulose, hemicellulose, pectin and lignin). Consequently, fungi that grow on koala faeces hold high potential as sources of enzymes for efficient degradation of plant biomass.  – Thirty-seven fungal strains were isolated from koala faeces, identified, and screened for enzyme activities using agar plate assays; over two-thirds of the isolates secreted xylanases, endoglucanases, ligninases and proteases, and over one-third secreted amylases, mannanases and tannases. The enzyme activities of seven isolates were comprehensively characterised using liquid cultures, liquid enzyme assays and zymography. Two isolates, Gelasinospora cratophora A10 and Trichoderma atroviride A2, were high secretors of protein and heat-tolerant enzymes. The lipase(s) from Mariannaea camptospora A11 sustained activity at cool temperatures. The xylanase(s), mannanase(s), endoglucanase(s) and β-glucosidase(s) of Doratomyces stemonitis C8 displayed optimal activities under neutral to alkaline conditions. Some of the enzymes hold potential for application in the production of paper, textiles, detergents and ethanol-based biofuels.  – Finally, the secretome of D. stemonitis C8 was studied by gel electrophoresis and mass spectrometry. As the genome of D. stemonitis has not been sequenced, the secretome analysis required cross-species identification and de novo sequencing; furthermore, a new technique was developed to identify proteins directly from zymogram gels by mass spectrometry. In the first secretome analysis of a coprophilous fungus, a complex array of enzymes integral to plant biomass degradation was identified, including enzymes that could be of value to industry in the future.%%%%%%%%1 online resource (243 p.) col. ill" @default.
• W2465461191 created "2016-07-22" @default.
• W2465461191 creator A5090205338 @default.
• W2465461191 date "2011-01-01" @default.
• W2465461191 modified "2023-09-26" @default.
• W2465461191 title "Enzyme activities of fungi isolated from koala faeces" @default.
• W2465461191 hasPublicationYear "2011" @default.
• W2465461191 type Work @default.
• W2465461191 sameAs 2465461191 @default.
• W2465461191 citedByCount "0" @default.
• W2465461191 crossrefType "journal-article" @default.
• W2465461191 hasAuthorship W2465461191A5090205338 @default.
• W2465461191 hasConcept C170835558 @default.
• W2465461191 hasConcept C172854840 @default.
• W2465461191 hasConcept C181199279 @default.
• W2465461191 hasConcept C2776073808 @default.
• W2465461191 hasConcept C2779251873 @default.
• W2465461191 hasConcept C2779678110 @default.
• W2465461191 hasConcept C2779697368 @default.
• W2465461191 hasConcept C28212737 @default.
• W2465461191 hasConcept C55493867 @default.
• W2465461191 hasConcept C59822182 @default.
• W2465461191 hasConcept C61716771 @default.
• W2465461191 hasConcept C86803240 @default.
• W2465461191 hasConcept C89423630 @default.
• W2465461191 hasConceptScore W2465461191C170835558 @default.
• W2465461191 hasConceptScore W2465461191C172854840 @default.
• W2465461191 hasConceptScore W2465461191C181199279 @default.
• W2465461191 hasConceptScore W2465461191C2776073808 @default.
• W2465461191 hasConceptScore W2465461191C2779251873 @default.
• W2465461191 hasConceptScore W2465461191C2779678110 @default.
• W2465461191 hasConceptScore W2465461191C2779697368 @default.
• W2465461191 hasConceptScore W2465461191C28212737 @default.
• W2465461191 hasConceptScore W2465461191C55493867 @default.
• W2465461191 hasConceptScore W2465461191C59822182 @default.
• W2465461191 hasConceptScore W2465461191C61716771 @default.
• W2465461191 hasConceptScore W2465461191C86803240 @default.
• W2465461191 hasConceptScore W2465461191C89423630 @default.
• W2465461191 hasLocation W24654611911 @default.
• W2465461191 hasOpenAccess W2465461191 @default.
• W2465461191 hasPrimaryLocation W24654611911 @default.
• W2465461191 hasRelatedWork W1975259467 @default.
• W2465461191 hasRelatedWork W1999229631 @default.
• W2465461191 hasRelatedWork W2008293852 @default.
• W2465461191 hasRelatedWork W2009559962 @default.
• W2465461191 hasRelatedWork W2016368845 @default.
• W2465461191 hasRelatedWork W2034358571 @default.
• W2465461191 hasRelatedWork W2040727825 @default.
• W2465461191 hasRelatedWork W2041369509 @default.
• W2465461191 hasRelatedWork W2147090905 @default.
• W2465461191 hasRelatedWork W2158133119 @default.
• W2465461191 hasRelatedWork W2191367773 @default.
• W2465461191 hasRelatedWork W2319422797 @default.
• W2465461191 hasRelatedWork W2466480109 @default.
• W2465461191 hasRelatedWork W2566756033 @default.
• W2465461191 hasRelatedWork W257328673 @default.
• W2465461191 hasRelatedWork W2956539302 @default.
• W2465461191 hasRelatedWork W2981092472 @default.
• W2465461191 hasRelatedWork W3048804993 @default.
• W2465461191 hasRelatedWork W3146372163 @default.
• W2465461191 hasRelatedWork W1925242685 @default.
• W2465461191 isParatext "false" @default.
• W2465461191 isRetracted "false" @default.
• W2465461191 magId "2465461191" @default.
• W2465461191 workType "article" @default.