Matches in SemOpenAlex for { <https://semopenalex.org/work/W2465488908> ?p ?o ?g. }
- W2465488908 abstract "Significance One of the biggest challenges in environmental microbiology is to determine the activity of uncultured cells directly in their habitat. We report on the application of bioorthogonal noncanonical amino acid tagging (BONCAT), a high-throughput approach to detecting protein synthesis in individual cells by fluorescence staining, on deep-sea methane seep sediments. By combining BONCAT with fluorescence in situ hybridization, we visualized active archaeal−bacterial consortia catalyzing the anaerobic oxidation of methane. We further developed a novel approach that combines BONCAT with fluorescence-activated cell sorting (FACS) to separate translationally active cells from complex samples. BONCAT-FACS enabled us to directly link the identities of anaerobic methane-oxidizing archaea with their partner bacteria for individual active consortia, uncovering previously unknown interactions between these archaea and Verrucomicrobia ." @default.
- W2465488908 created "2016-07-22" @default.
- W2465488908 creator A5006298901 @default.
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- W2465488908 creator A5071685676 @default.
- W2465488908 creator A5080464756 @default.
- W2465488908 creator A5086417208 @default.
- W2465488908 date "2016-06-28" @default.
- W2465488908 modified "2023-10-11" @default.
- W2465488908 title "Visualizing in situ translational activity for identifying and sorting slow-growing archaeal−bacterial consortia" @default.
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