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• W2465796747 abstract "The development of molecular markers in kiwifruit followed the advances in molecular biology techniques. After occasional use of RFLP (restriction fragment length polymorphism) markers in the 1990s, most markers developed in kiwifruit were based on PCR (polymerase chain reaction). RAPD (random amplified polymorphic DNA) were followed by AFLPs (amplified fragment length polymorphisms), used mainly for the saturation of linkage maps. In the meantime, microsatellites or SSRs (simple sequence repeats) came to the fore and were extensively used for genotyping germplasm accessions and to produce linkage maps, where SSRs anchored markers for pairing parental maps of the pseudo-testcrosses and the identification of homologous linkage groups in maps produced from different cross populations. Four linkage maps for kiwifruit have been published: the first two based on SSR markers and the second two based mainly or exclusively on SNP markers identified and mapped at the same time through the approach of ‘genotyping-by-sequencing.’ These last, highly saturated, maps were produced to assist the genome-sequencing project, by anchoring and orientating scaffolds and contigs of the assembly. The linkage maps, in particular that published by the New Zealand Institute for Plant & Food Research based on 636 markers, mainly SSRs, helped in the mapping of several traits, such as gender that was mapped in the subtelomeric region of linkage group 17, corresponding to the pseudo-molecule number 25 of the genome assembly." @default.
• W2465796747 created "2016-07-22" @default.
• W2465796747 creator A5000911912 @default.
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• W2465796747 date "2016-01-01" @default.
• W2465796747 modified "2023-09-27" @default.
• W2465796747 title "Markers, Maps, and Marker-Assisted Selection" @default.
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• W2465796747 doi "https://doi.org/10.1007/978-3-319-32274-2_7" @default.
• W2465796747 hasPublicationYear "2016" @default.
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