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- W2465878341 abstract "Ochratoxin A (OTA), the mycotoxin produced by species of Aspergilli, specifically Aspergillus carbonarius and Aspergillus ochraceus, and Penicillium verrucosum have harmful effects on humans, animals, and crops that result in illnesses and economic losses. Therefore, this study has been undertaken the cytotoxic effects of ochratoxin A of the four moulds extracts which has been used in this study in two human cell lines(Hfl-1 and Hep-2). Control experiment (uncontaminated solid substrate extract) showed negligible cytotoxic effects on both cell lines after 72 hr exposure. All mould (OTA) extracts examined showed a cytotoxic effect on both cell lines, although it is possible that other compounds in the extracts may have contributed to the cytotoxic effects. Effect of the toxin extracts on the cell lines increased with incubation time. Aspergillus ochraceus J002 and A. ochraceus strain CBS 588.68 showed higher cytotoxic effects on Hfl-1 than A. carbonarius J001 and P. verrucosum strain IMI 285522 whereas on Hep-2, A. carbonarius J001 and A. ochraceus strain CBS 588.68 showed higher cytotoxic effects than A. ochraceus J002 and P. verrucosum IMI 285522. IC50 ranged between 1.37 to 38.87 using Hfl-1 cells and 1.94 to 50.0 using Hep-2 cells. IC50 values by Aspergillus ochraceus strain CBS 588.68 was not determined after 72hr, this may have been due to higher concentration of OTA in the extract than suspected." @default.
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- W2465878341 date "2016-07-10" @default.
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- W2465878341 title "Studies on Cytotoxic Effects of Ochratoxin A Extracts on Human Cell Lines (Hfl-1 And Hep-2)" @default.
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- W2465878341 doi "https://doi.org/10.20546/ijcmas.2016.507.005" @default.
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