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- W2466281853 abstract "AbstractIn this chapter we describe the steps needed for reconstructing the three-dimensional structure of a macromolecular complex starting from its projections collected in electron micrographs. The concepts are shown through the use of Xmipp 3.0, a software suite specifically designed for the image processing of biological structures imaged with electron or X-ray microscopy. We illustrate the image processing workflow by applying it to the images of Bovine Papilloma virus published in Wolf et al. (Proc Natl Acad Sci USA 107:6298–6303, 2010). We show that in the case of high-quality, homogeneous datasets with a priori knowledge about the initial volume, we can have a high-resolution 3D reconstruction in less than 1 day using a computer cluster with only 32 processors.Key wordsSingle particle analysisElectron microscopyImage processing3D reconstructionWorkflows" @default.
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- W2466281853 date "2012-09-18" @default.
- W2466281853 modified "2023-10-16" @default.
- W2466281853 title "Semiautomatic, High-Throughput, High-Resolution Protocol for Three-Dimensional Reconstruction of Single Particles in Electron Microscopy" @default.
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- W2466281853 doi "https://doi.org/10.1007/978-1-62703-137-0_11" @default.
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