FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2466734964> ?p ?o ?g. }

• W2466734964 endingPage "230" @default.
• W2466734964 startingPage "224" @default.
• W2466734964 abstract "In this paper, a label-free, highly sensitive and simple assay for one step detection of protein kinase (PKA) activity and inhibition that avoids the fluorescent dye process has been established. The detection was based on the fluorescence (FL) quenching of peptide-Ag nanoclusters (Ag NCs) caused by antibody modified Au nanoparticles (anti-Au NPs) via fluorescence resonance energy transfer (FRET). With PKA and adenosine 5′-triphosphate (ATP) introduced, the substrate peptide of Ag NCs could react with PKA via targeted phosphorylation, and followed by the linking interactions between peptide-Ag NCs and anti-Au NPs. According to the fluorescence quenching of Ag NCs, the activity of protein kinase can be facilely monitored in the range of 0.1–2000 mU/μL with high sensitivity. The detection limit for PKA is 0.039 mU/μL. We further explored the inhibitory effect of H-89 for protein kinase activity. The developed method was also applied to the investigation of drug-induced PKA activation in HeLa cells, which provides a promising means for screening of kinase-related drugs and the clinical diagnosis of disease." @default.
• W2466734964 created "2016-07-22" @default.
• W2466734964 creator A5059717344 @default.
• W2466734964 creator A5067113392 @default.
• W2466734964 creator A5073216396 @default.
• W2466734964 date "2016-09-01" @default.
• W2466734964 modified "2023-10-09" @default.
• W2466734964 title "A label-free and sensitive fluorescent assay for one step detection of protein kinase activity and inhibition" @default.
• W2466734964 cites W1886936963 @default.
• W2466734964 cites W1966735644 @default.
• W2466734964 cites W1991285913 @default.
• W2466734964 cites W1991382853 @default.
• W2466734964 cites W1991544841 @default.
• W2466734964 cites W1999575246 @default.
• W2466734964 cites W2003495102 @default.
• W2466734964 cites W2011660201 @default.
• W2466734964 cites W2017248460 @default.
• W2466734964 cites W2030205108 @default.
• W2466734964 cites W2045028530 @default.
• W2466734964 cites W2057317821 @default.
• W2466734964 cites W2059888301 @default.
• W2466734964 cites W2072734966 @default.
• W2466734964 cites W2090110748 @default.
• W2466734964 cites W2095068497 @default.
• W2466734964 cites W2099279744 @default.
• W2466734964 cites W2131665703 @default.
• W2466734964 cites W2135545928 @default.
• W2466734964 cites W2135917319 @default.
• W2466734964 cites W2161313073 @default.
• W2466734964 cites W2162256094 @default.
• W2466734964 cites W2236469530 @default.
• W2466734964 cites W2321861141 @default.
• W2466734964 cites W2324288165 @default.
• W2466734964 cites W2328681900 @default.
• W2466734964 cites W2332562190 @default.
• W2466734964 cites W2335677937 @default.
• W2466734964 cites W2335695938 @default.
• W2466734964 doi "https://doi.org/10.1016/j.aca.2016.06.007" @default.
• W2466734964 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27543031" @default.
• W2466734964 hasPublicationYear "2016" @default.
• W2466734964 type Work @default.
• W2466734964 sameAs 2466734964 @default.
• W2466734964 citedByCount "18" @default.
• W2466734964 countsByYear W24667349642017 @default.
• W2466734964 countsByYear W24667349642018 @default.
• W2466734964 countsByYear W24667349642019 @default.
• W2466734964 countsByYear W24667349642020 @default.
• W2466734964 countsByYear W24667349642021 @default.
• W2466734964 countsByYear W24667349642022 @default.
• W2466734964 crossrefType "journal-article" @default.
• W2466734964 hasAuthorship W2466734964A5059717344 @default.
• W2466734964 hasAuthorship W2466734964A5067113392 @default.
• W2466734964 hasAuthorship W2466734964A5073216396 @default.
• W2466734964 hasConcept C111368507 @default.
• W2466734964 hasConcept C119128265 @default.
• W2466734964 hasConcept C121332964 @default.
• W2466734964 hasConcept C121745418 @default.
• W2466734964 hasConcept C12554922 @default.
• W2466734964 hasConcept C127313418 @default.
• W2466734964 hasConcept C178790620 @default.
• W2466734964 hasConcept C184235292 @default.
• W2466734964 hasConcept C185592680 @default.
• W2466734964 hasConcept C21951064 @default.
• W2466734964 hasConcept C2777289219 @default.
• W2466734964 hasConcept C2778402822 @default.
• W2466734964 hasConcept C2779281246 @default.
• W2466734964 hasConcept C2779564974 @default.
• W2466734964 hasConcept C43617362 @default.
• W2466734964 hasConcept C55493867 @default.
• W2466734964 hasConcept C62520636 @default.
• W2466734964 hasConcept C86803240 @default.
• W2466734964 hasConcept C91881484 @default.
• W2466734964 hasConcept C96305047 @default.
• W2466734964 hasConcept C97029542 @default.
• W2466734964 hasConceptScore W2466734964C111368507 @default.
• W2466734964 hasConceptScore W2466734964C119128265 @default.
• W2466734964 hasConceptScore W2466734964C121332964 @default.
• W2466734964 hasConceptScore W2466734964C121745418 @default.
• W2466734964 hasConceptScore W2466734964C12554922 @default.
• W2466734964 hasConceptScore W2466734964C127313418 @default.
• W2466734964 hasConceptScore W2466734964C178790620 @default.
• W2466734964 hasConceptScore W2466734964C184235292 @default.
• W2466734964 hasConceptScore W2466734964C185592680 @default.
• W2466734964 hasConceptScore W2466734964C21951064 @default.
• W2466734964 hasConceptScore W2466734964C2777289219 @default.
• W2466734964 hasConceptScore W2466734964C2778402822 @default.
• W2466734964 hasConceptScore W2466734964C2779281246 @default.
• W2466734964 hasConceptScore W2466734964C2779564974 @default.
• W2466734964 hasConceptScore W2466734964C43617362 @default.
• W2466734964 hasConceptScore W2466734964C55493867 @default.
• W2466734964 hasConceptScore W2466734964C62520636 @default.
• W2466734964 hasConceptScore W2466734964C86803240 @default.
• W2466734964 hasConceptScore W2466734964C91881484 @default.
• W2466734964 hasConceptScore W2466734964C96305047 @default.
• W2466734964 hasConceptScore W2466734964C97029542 @default.
• W2466734964 hasFunder F4320321001 @default.
• W2466734964 hasLocation W24667349641 @default.
• W2466734964 hasLocation W24667349642 @default.

• next