Matches in SemOpenAlex for { <https://semopenalex.org/work/W2466962336> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2466962336 endingPage "217" @default.
- W2466962336 startingPage "213" @default.
- W2466962336 abstract "Yarrowia lipolytica is a nonconventional model micro-organism with multiple biotechnological applications. It is also considered to be an excellent producer for lipase. Genome survey shows that Y. lipolytica possesses various paralogs of genes coding for extracellular, cell-bound, and intracellular lipolytic enzymes. However, little structural information on these isoenzymes is available. With the aim to facilitate crystal structure solution of Lip8, one of the most valuable lipases from Y. lipolytica, a less conventional protein expression technique-selenomethionyl protein expression was used to produce recombinant selenomethionine (SeMet)-Lip8 in Escherichia coli. Finally, three Met residues of Lip8 were all substituted with SeMet. A total of 72 mg of SeMet-Lip8 was obtained from a liter of the SeMet medium. Using sodium acetate as a precipitant and ammonium sulfate as an additive, crystals of the SeMet-Lip8 with 1.9 Å were successfully cultured through hanging-drop vapor diffusion method. The estimated crystal dimensions were 0.11 × 0.11 × 0.14 mm2. The crystal belonged to the space group I4 with unit cell parameters a = b = 128.87 Å, c = 171.77 Å, α = β = γ = 90°. It is the second member of lipase crystal family from Y. lipolytica. This work will provide a platform for further studying lipases from a structural insight." @default.
- W2466962336 created "2016-07-22" @default.
- W2466962336 creator A5002338980 @default.
- W2466962336 creator A5030398519 @default.
- W2466962336 creator A5059170837 @default.
- W2466962336 creator A5066448103 @default.
- W2466962336 date "2018-03-16" @default.
- W2466962336 modified "2023-10-16" @default.
- W2466962336 title "Expression, purification, crystallization, and diffraction analysis of a selenomethionyl lipase Lip8 from <i>Yarrowia lipolytica</i>" @default.
- W2466962336 cites W1595115988 @default.
- W2466962336 cites W1965582391 @default.
- W2466962336 cites W1980340464 @default.
- W2466962336 cites W1982642101 @default.
- W2466962336 cites W2000902017 @default.
- W2466962336 cites W2004603941 @default.
- W2466962336 cites W2023493858 @default.
- W2466962336 cites W2029584951 @default.
- W2466962336 cites W2031138420 @default.
- W2466962336 cites W2039790529 @default.
- W2466962336 cites W2056077497 @default.
- W2466962336 cites W2059384131 @default.
- W2466962336 cites W2066973013 @default.
- W2466962336 cites W2074180965 @default.
- W2466962336 cites W2091621302 @default.
- W2466962336 cites W2092427077 @default.
- W2466962336 cites W2137579444 @default.
- W2466962336 cites W2143132058 @default.
- W2466962336 cites W2154557455 @default.
- W2466962336 cites W2165360475 @default.
- W2466962336 doi "https://doi.org/10.1080/10826068.2016.1188316" @default.
- W2466962336 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27380164" @default.
- W2466962336 hasPublicationYear "2018" @default.
- W2466962336 type Work @default.
- W2466962336 sameAs 2466962336 @default.
- W2466962336 citedByCount "4" @default.
- W2466962336 countsByYear W24669623362018 @default.
- W2466962336 countsByYear W24669623362019 @default.
- W2466962336 countsByYear W24669623362020 @default.
- W2466962336 countsByYear W24669623362022 @default.
- W2466962336 crossrefType "journal-article" @default.
- W2466962336 hasAuthorship W2466962336A5002338980 @default.
- W2466962336 hasAuthorship W2466962336A5030398519 @default.
- W2466962336 hasAuthorship W2466962336A5059170837 @default.
- W2466962336 hasAuthorship W2466962336A5066448103 @default.
- W2466962336 hasConcept C104317684 @default.
- W2466962336 hasConcept C181199279 @default.
- W2466962336 hasConcept C185592680 @default.
- W2466962336 hasConcept C2779490540 @default.
- W2466962336 hasConcept C2779697368 @default.
- W2466962336 hasConcept C28406088 @default.
- W2466962336 hasConcept C40767141 @default.
- W2466962336 hasConcept C547475151 @default.
- W2466962336 hasConcept C55493867 @default.
- W2466962336 hasConcept C86803240 @default.
- W2466962336 hasConceptScore W2466962336C104317684 @default.
- W2466962336 hasConceptScore W2466962336C181199279 @default.
- W2466962336 hasConceptScore W2466962336C185592680 @default.
- W2466962336 hasConceptScore W2466962336C2779490540 @default.
- W2466962336 hasConceptScore W2466962336C2779697368 @default.
- W2466962336 hasConceptScore W2466962336C28406088 @default.
- W2466962336 hasConceptScore W2466962336C40767141 @default.
- W2466962336 hasConceptScore W2466962336C547475151 @default.
- W2466962336 hasConceptScore W2466962336C55493867 @default.
- W2466962336 hasConceptScore W2466962336C86803240 @default.
- W2466962336 hasIssue "3" @default.
- W2466962336 hasLocation W24669623361 @default.
- W2466962336 hasLocation W24669623362 @default.
- W2466962336 hasOpenAccess W2466962336 @default.
- W2466962336 hasPrimaryLocation W24669623361 @default.
- W2466962336 hasRelatedWork W1965618318 @default.
- W2466962336 hasRelatedWork W1972665833 @default.
- W2466962336 hasRelatedWork W2004603941 @default.
- W2466962336 hasRelatedWork W2016628627 @default.
- W2466962336 hasRelatedWork W2021012837 @default.
- W2466962336 hasRelatedWork W2025468017 @default.
- W2466962336 hasRelatedWork W2038994242 @default.
- W2466962336 hasRelatedWork W2060127218 @default.
- W2466962336 hasRelatedWork W349977252 @default.
- W2466962336 hasRelatedWork W4289920555 @default.
- W2466962336 hasVolume "48" @default.
- W2466962336 isParatext "false" @default.
- W2466962336 isRetracted "false" @default.
- W2466962336 magId "2466962336" @default.
- W2466962336 workType "article" @default.