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- W2467059250 abstract "To clone human epidermal growth factor receptor (HER2) gene and establish a MCF-7 cell line with stable co-expression of HER2 and estrogen receptor (ER).The full-length HER2 gene fragment was amplified by RT-PCR from the total RNA extracted from human breast cancer cell line SKBR-3 which over-expressed HER2 protein. The gene fragment was then inserted into the vector pcDNA3.1 to construct the recombinant expression plasmid pcDNA3.1-HER2, which was identified by sequence analysis and transfected into ER-expressing MCF-7 cells via lipofectamine. The positive cell clones were obtained after G418 selection. The MCF-7 cells transfected with HER2-cDNA was assayed by immunocytochemistry, flow cytometry and Western blotting.DNA sequencing results showed that HER2 gene was exactly consistent with the sequence reported in GenBank. The MCF-7 cells transfected with HER2 could highly express HER2 protein as shown by immunocytochemistry, flow cytometry and Western blotting.HER2 gene has been cloned successfully and the MCF-7 cell line co-expressing HER2 and ER is obtained." @default.
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- W2467059250 date "2005-10-01" @default.
- W2467059250 modified "2023-09-28" @default.
- W2467059250 title "[Cloning and expression of the HER2 gene in MCF-7 cells]." @default.
- W2467059250 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16234104" @default.
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