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- W2467218493 abstract "ABSTRACTTo understand the effects of the eIF-5A gene in response to abiotic stress, the 909 bp full-length cDNA of eIF-5A, including a 480 bp open reading frame encoding 159 amino-acid (aa) residues, was isolated from the leaf of herbal plant (Apocynum venetum) by rapid-amplification of cDNA ends. The deduced molecular weight of the encoding protein was 17.48 kDa with a theoretical pI of 5.61 and predicted no signal peptide. Real-time polymerase chain reaction analysis revealed that AveIF-5A gene expression was induced by cold, salt and drought stress. To determine the biological function of this gene, recombinant plasmids expressing AveIF-5A and AveIF-5A1 (86–156 aa truncated polypeptide) were used to transform Escherichia coli. The analysis of the growth curves of recombinant E. coli revealed that AveIF-5A and AveIF-5A1 improved the resistance of transformed E. coli to low-temperature, drought and salt stress. These results could provide experimental evidence of the function of the AveIF-5A gene as a va..." @default.
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- W2467218493 date "2016-05-09" @default.
- W2467218493 modified "2023-09-24" @default.
- W2467218493 title "Cloning and expression analysis of eIF-5A gene in Apocynum venetum" @default.
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- W2467218493 doi "https://doi.org/10.1080/13102818.2016.1172944" @default.
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