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- W2467610055 abstract "This chapter provides protocols to measure the reversible permeabilization of mast cells by streptolysin O (SLO) and to follow SLO-induced activation of mast cells by monitoring degranulation, activation of mitogen-activated protein kinases, and production of tumor necrosis factor-alpha. A method that uses SLO to deliver molecules into the cytosol of living cells also is described. Furthermore, we outline a procedure to measure the activation of nuclear factor-kappaB by lipopolysaccharide and ionomycin using transfection of mast cells with reporter genes by electroporation. These protocols should be widely applicable in mast cell research." @default.
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- W2467610055 date "2005-08-18" @default.
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- W2467610055 title "Activation of Mast Cells by Streptolysin O and Lipopolysaccharide" @default.
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- W2467610055 doi "https://doi.org/10.1385/1-59259-967-2:393" @default.
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