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- W2467614901 abstract "Background: Clindamycin resistance among clinically significant staphylococcal isolates is now increasing. Routine testing of staphylococcal isolates for inducible clindamycin resistance (ICR) is advocated by the Clinical Laboratory Standards Institute (CLSI). Automated system Vitek 2 offers a panel that detects inducible clindamycin resistance directly. it is easy and more cost-effective than the more labor-intensive CLSI reference methods. Objectives: The aim of this study was to assess the performance of Vitek-2 system for ICR detection against the reference D-test, as well as to detect the presence of erm-A and erm-C genes among these isolates. Methodology: 22 clinical staphylococcal isolates (15 Staph aureus and 7 coagulase negative Staph (CoNS) were examined for ICR both by D- test and Vitek-2 system. multiplex PCR was performed for the isolates to detect ermA and ermC genes. Results: Out of the 22 isolates, ICR was detected by Vitek-2 in 6 isolates (27.7%). five of the 6 isolates were confirmed by D-test. sensitivity, specificity, positive and negative predictive values were calculated as 100%, 94.1%, 83.33% and 100% respectively. ermC gene was detected in 20/22 isolates, 5 isolates were positive for both genes. Conclusion: Vitek-2 is considered a potentially reliable test for detection of ICR, further studies are recommended on large number of isolates." @default.
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- W2467614901 date "2015-01-01" @default.
- W2467614901 modified "2023-09-22" @default.
- W2467614901 title "Performance of Vitek-2 System for Detection of Inducible Clindamycin Resistance among Clinical Isolates of Staphylococci in Comparison to the D-Test" @default.
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- W2467614901 doi "https://doi.org/10.12816/0030401" @default.
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