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- W2468154867 abstract "Abstract Lack of resistance to pink snow mould ( Microdochium nivale ) is a major constraint for adaptation of perennial ryegrass ( Lolium perenne L.) to continental regions with long-lasting snow cover at higher latitudes. Almost all investigations of genetic variation in resistance have been performed using cold acclimated plants. However, there may be variation in resistance mechanisms that are functioning independently of cold acclimation. In this study our aim was to identify candidate genes involved in such resistance mechanisms. We first characterized variation in resistance to M. nivale among non-acclimated genotypes from the Norwegian cultivar ‘Fagerlin’ based on relative regrowth and fungal quantification by real-time qPCR. One resistant and one susceptible genotype were selected for transcriptome analysis using paired-end sequencing by Illumina Hiseq 2000. Transcriptome profiles, GO enrichment and KEGG pathway analysis indicate that defense response related genes are differentially expressed between the resistant and the susceptible genotype. A significant up-regulation of defense related genes, as well as genes involved in cell wall cellulose metabolic processes and aryl-alcohol dehydrogenase (NADP+) activity, was observed in the resistant genotype. The candidate genes identified in this study might be potential molecular marker resources for breeding perennial ryegrass cultivars with improved resistance to pink snow mould." @default.
- W2468154867 created "2016-07-22" @default.
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- W2468154867 date "2016-06-27" @default.
- W2468154867 modified "2023-09-26" @default.
- W2468154867 title "Global transcriptome changes in perennial ryegrass during early infection by pink snow mould" @default.
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- W2468154867 doi "https://doi.org/10.1038/srep28702" @default.
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