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- W2468345382 abstract "Objective To investigate the role of heme oxygenase-1 (HO-1) in the oxidative stress damage of intestinal mucosa barrier disruption in patients with malignant obstructive jaundice (MOJ). Methods Fifteen jaundiced patients with malignant biliary obstruction undergoing endoscopic retrograde cholangiopancreatography ( ERCP) examination or treatment were enrolled. The control group was comprised of 10 healthy subjects with gastroscopy and 10 patients with non-jaundiced biliary obstruction. Patients were subjected to duodenal biopsy to assess the intestinal oxidative stress as estimated by lipid peroxidation ( malondialdehyde) and activity of superoxide dismutase (SOD). Apoptosis of epithelial cell was examined by TdT-mediated dUTP-biotin nick end labeling. Immunohislochemistry and Western blotting were employed to examine the distribution and expression of HO-1 proteins in intestinal mucosa. Results MOJ jaundiced patients presented high levels of intestinal oxidative stress with a significantly increased level of lipid peroxidation[(1. 79 ±0. 24) vs (1.09 ±0.28) vs (1. 18 ±0.32) nmol ·mg~(-1) · prot~(-1), P=0. 041] and a decreased SOD activity [(303±10)vs (398 ±11) vs (406 ± 11 )nmol · mg~(-1) · prot~(-1), P=0.017]. The apoptotic rate of intestinal epithelial cells was significantly higher in jaundiced group than in non-jaundiced control group. Apoptotic index was (69. 1 ± 5. 9) % , (28. 6 ± 3. 5) % and (10. 2 ± 2. 5) % respectively (P<0. 01). The staining of HO-1 was predominantly localized in cytoplasm. In jaundiced patients, HO-1 was obviously elevated than those in the control group (HO-1 optical density 0. 28 ±0. 04 vs 0. 20 ±0. 04 vs 0. 13 ±0. 05) ( P < 0. 01 ) . Similar outcomes were obtained by quantitative analysis of Western blotting images [HO-1/GAPDH (10.7±0.7)% vs (7.6±0.5)% vs (3. 9 ±0. 4)% ,P <0. 01]. Conclusion MOJ induces intestinal oxidative stress and it may be a key contributing factor to intestinal barrier failure in the patient population. HO-1 protein level is rising with the progression of obstruction. Perhaps HO-1 has a protective effect upon MOJ through anli-oxidative damage.Key words: Jaundice; obstructive; Heme oxygenase-1; Oxidative stress; Lipid peroxidation" @default.
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- W2468345382 date "2009-12-29" @default.
- W2468345382 modified "2023-09-27" @default.
- W2468345382 title "[Heme oxygenase-1 overexpression in patients with malignant obstructive jaundice]." @default.
- W2468345382 doi "https://doi.org/10.3760/cma.j.issn.0376-2491.2009.48.006" @default.
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