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• W2468375317 endingPage "e2287" @default.
• W2468375317 startingPage "e2287" @default.
• W2468375317 abstract "We previously reported that renal clear cell carcinoma cells (RCC) express both tumor necrosis factor receptor (TNFR)-1 and -2, but that, in organ culture, a TNF mutein that only engages TNFR1, but not TNFR2, causes extensive cell death. Some RCC died by apoptosis based on detection of cleaved caspase 3 in a minority TUNEL-positive cells but the mechanism of death in the remaining cells was unexplained. Here, we underpin the mechanism of TNFR1-induced cell death in the majority of TUNEL-positive RCC cells, and show that they die by necroptosis. Malignant cells in high-grade tumors displayed threefold to four fold higher expression of both receptor-interacting protein kinase (RIPK)1 and RIPK3 compared with non-tumor kidney tubular epithelium and low-grade tumors, but expression of both enzymes was induced in lower grade tumors in organ culture in response to TNFR1 stimulation. Furthermore, TNFR1 activation induced significant MLKL(Ser358) and Drp1(Ser616) phosphorylation, physical interactions in RCC between RIPK1-RIPK3 and RIPK3-phospho-MLKL(Ser358), and coincidence of phospho-MLKL(ser358) and phospho-Drp1(Ser616) at mitochondria in TUNEL-positive RCC. A caspase inhibitor only partially reduced the extent of cell death following TNFR1 engagement in RCC cells, whereas three inhibitors, each targeting a different step in the necroptotic pathway, were much more protective. Combined inhibition of caspases and necroptosis provided additive protection, implying that different subsets of cells respond differently to TNF-α, the majority dying by necroptosis. We conclude that most high-grade RCC cells express increased amounts of RIPK1 and RIPK3 and are poised to undergo necroptosis in response to TNFR1 signaling." @default.
• W2468375317 created "2016-07-22" @default.
• W2468375317 creator A5020505790 @default.
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• W2468375317 creator A5076147739 @default.
• W2468375317 date "2016-06-01" @default.
• W2468375317 modified "2023-10-17" @default.
• W2468375317 title "Tubular epithelial cells in renal clear cell carcinoma express high RIPK1/3 and show increased susceptibility to TNF receptor 1-induced necroptosis" @default.
• W2468375317 cites W1537667127 @default.
• W2468375317 cites W1548775239 @default.
• W2468375317 cites W1562692846 @default.
• W2468375317 cites W1568398137 @default.
• W2468375317 cites W1666754568 @default.
• W2468375317 cites W1778352924 @default.
• W2468375317 cites W1963848568 @default.
• W2468375317 cites W1969323641 @default.
• W2468375317 cites W1976647852 @default.
• W2468375317 cites W1979032223 @default.
• W2468375317 cites W1981682822 @default.
• W2468375317 cites W1987186266 @default.
• W2468375317 cites W1987213099 @default.
• W2468375317 cites W1987258027 @default.
• W2468375317 cites W1992496728 @default.
• W2468375317 cites W1992754215 @default.
• W2468375317 cites W1993613360 @default.
• W2468375317 cites W1997417360 @default.
• W2468375317 cites W2007508922 @default.
• W2468375317 cites W2012985988 @default.
• W2468375317 cites W2013246554 @default.
• W2468375317 cites W2016716143 @default.
• W2468375317 cites W2016883314 @default.
• W2468375317 cites W201697683 @default.
• W2468375317 cites W2018720554 @default.
• W2468375317 cites W2020987391 @default.
• W2468375317 cites W2021256410 @default.
• W2468375317 cites W2034006241 @default.
• W2468375317 cites W2038096193 @default.
• W2468375317 cites W2039426833 @default.
• W2468375317 cites W2043861387 @default.
• W2468375317 cites W2044152887 @default.
• W2468375317 cites W2045171661 @default.
• W2468375317 cites W2047271635 @default.
• W2468375317 cites W2048588387 @default.
• W2468375317 cites W2052605153 @default.
• W2468375317 cites W2054389254 @default.
• W2468375317 cites W2055699906 @default.
• W2468375317 cites W2057123987 @default.
• W2468375317 cites W2058436322 @default.
• W2468375317 cites W2059009119 @default.
• W2468375317 cites W2060204808 @default.
• W2468375317 cites W2063211255 @default.
• W2468375317 cites W2065018825 @default.
• W2468375317 cites W2068222437 @default.
• W2468375317 cites W2070143715 @default.
• W2468375317 cites W2073581978 @default.
• W2468375317 cites W2082870439 @default.
• W2468375317 cites W2085701360 @default.
• W2468375317 cites W2087232320 @default.
• W2468375317 cites W2091363184 @default.
• W2468375317 cites W2096297372 @default.
• W2468375317 cites W2098510786 @default.
• W2468375317 cites W2099583097 @default.
• W2468375317 cites W2102073127 @default.
• W2468375317 cites W2104028914 @default.
• W2468375317 cites W2107411016 @default.
• W2468375317 cites W2110796758 @default.
• W2468375317 cites W2113198849 @default.
• W2468375317 cites W2114668404 @default.
• W2468375317 cites W2117163370 @default.
• W2468375317 cites W2120626244 @default.
• W2468375317 cites W2124396497 @default.
• W2468375317 cites W2128854284 @default.
• W2468375317 cites W2132161879 @default.
• W2468375317 cites W2139552156 @default.
• W2468375317 cites W2140883369 @default.
• W2468375317 cites W2144173662 @default.
• W2468375317 cites W2147551208 @default.
• W2468375317 cites W2152445095 @default.
• W2468375317 cites W2158589169 @default.
• W2468375317 cites W2158662692 @default.
• W2468375317 cites W2159190959 @default.
• W2468375317 cites W2159864138 @default.
• W2468375317 cites W2160651627 @default.
• W2468375317 cites W2161830466 @default.
• W2468375317 cites W2163728657 @default.
• W2468375317 cites W296161204 @default.
• W2468375317 cites W4242587027 @default.
• W2468375317 doi "https://doi.org/10.1038/cddis.2016.184" @default.
• W2468375317 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5108336" @default.
• W2468375317 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27362805" @default.
• W2468375317 hasPublicationYear "2016" @default.
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