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- W2468567756 abstract "Gelatinolytic activity induced by longtime incubation at 37 degrees C was found in extracts of rheumatoid synovial tissues. The enzyme activity was assessed by gelatin zymography and 3H-gelatin degradation assay. The observed enzyme had different characteristics from matrix metalloproteinases; it did not require metal ions for activity. However, metallocheltors blocked activation and addition of some metal ions restored the activation. The molecular size of the enzyme was changed time-dependently. The approximate molecular weight of the first enzyme produced by incubation was 65 kDa and it was converted to a broad size molecule with a molecular weight of 50 kDa after further incubation. Substrate specificity was detected for denatured collagen types I, II, III and IV." @default.
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- W2468567756 date "2000-01-01" @default.
- W2468567756 modified "2023-09-23" @default.
- W2468567756 title "Induction of a novel gelatinolytic activity in synovial tissue of patients with rheumatoid arthritis." @default.
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