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- W246867345 abstract "Three primers were designed according to the 3′ terminal sequence of apxⅣA gene of Actinobacillus pleuropneumoniae(APP) serotype 1 to develop a semi-nested PCR assay for detection of APP.Reaction conditions of the semi-nested PCR were optimized.The efficacy of detection was compared by using different sample preservation and DNA extraction methods.The developed semi-nested PCR could amplify unique band of approximately 250 bp in size from genomic DNA of all tested samples of APP strains,while no other band was observed from bacteria other than APP in porcine upper respiratory tract,indicating that the semi-nested PCR possessed good specificity.16.5 fg of genomic DNA was detected by the semi-nested PCR,and the sensitivity of the semi-nested PCR was 1 000 fold higher than that of the one-step PCR method.The products by the semi-nested PCR were always consistent for 5 times in reproducibility.The positive rate of clinical samples detected by the semi-nested PCR was higher than that by the bacterial isolation method.The preservation conditions and the extracting methods for DNA significantly affected the positive rate by the semi-nested PCR." @default.
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- W246867345 date "2009-01-01" @default.
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- W246867345 title "Development and application of a semi-nested PCR assay for detection of porcine Actinobacillus pleuropneumoniae." @default.
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