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- W2468752584 abstract "Imaging TOF-SIMS now has the potential to provide spatially resolved chemical information for a wide variety of molecules in biological tissue and single cells. Although early studies focused on low molecular weight fragment ions, the advent of polyatomic primary ion sources has opened detection schemes to a much wider range of molecules with molecular weights extending to greater than 1000 Da. In this regard, a number of workers have reported the distribution of drug molecules and of metabolites under various conditions. Here we discuss a number of challenges facing this field given the new measurement paradigms. Very different sample preparation measurement considerations emerge as the desired spatial resolution approaches 1 micron. At values above 1 micron, freeze drying methods appear to be successful and there are enough molecules in the probe area to detect higher mass species directly. For example, we have detected the molecular ion of more than 50 different lipids in brain tissue and in a lawn of cells, using ms/ms techniques to assign structure. Characterization of single cells becomes much more challenging, however, since there are many fewer molecules available for detection, and sample pretreatment to enhance ionization can move molecules around, reducing the effective lateral resolution. For these systems, preparation of frozen hydrated samples appears to be the best way to avoid artifacts. Moreover, since polyatomic projectiles now allow molecular depth profiling, use of frozen hydrated samples is the only way to preserve 3-dimensional structure of cells. Currently, using a conventional high performance imaging TOF-SIMS system, the sensitivity is on the limit of being able to detect the higher mass molecular ions. Several examples will be given to illustrate each of the above issues. The prospects for improving the sensitivity of this type of imaging will also be discussed." @default.
- W2468752584 created "2016-07-22" @default.
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- W2468752584 date "2012-01-01" @default.
- W2468752584 modified "2023-09-24" @default.
- W2468752584 title "Nanoscale Chemical Imaging of Biomaterials with Mass Spectrometry." @default.
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